AJP - GI AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 279: G483-G491, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oktar, B. K.
Right arrow Articles by Kurtel, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oktar, B. K.
Right arrow Articles by Kurtel, H.
Vol. 279, Issue 3, G483-G491, September 2000

Endothelin-1-induced PMN infiltration and mucosal dysfunction in the rat small intestine

Berna K. Oktar1, Tamer Coskun1, Ayhan Bozkurt1, Berrak Ç. Yegen1, Meral Yüksel2, Goncagül Haklar2, Serpil Bilsel2, Fehime Benli Aksungar2, Şule Çetinel3, D. Neil Granger4, and Hizir Kurtel1

Departments of 1 Physiology, 2 Biochemistry, and 3 Histology, Marmara University School of Medicine, 81326 Haydarpasa, İstanbul, Turkey; and 4 Department of Molecular and Cellular Physiology, Louisiana State University, Shreveport, Louisiana 71130

The objectives of this study were to characterize the effects of endothelin (ET)-1 on intestinal mucosal parameters and to assess the contribution of polymorphonuclear leukocytes (PMNs), intercellular adhesion molecule-1 (ICAM-1), and a platelet-activating factor (PAF) to the mucosal dysfunction induced by ET-1. Different concentrations of ET-1 (100, 200, and 400 pmol/kg) were infused into the superior mesenteric artery for 10 min, and tissue samples were obtained 30 min after terminating the infusion. ET-1 administration significantly elevated tissue myeloperoxidase activity, plasma carbonyl content, and tissue chemiluminescence intensity, indicating that ET-1 produces PMN infiltration and oxidant stress. Blood-to-lumen clearance of 51Cr-EDTA significantly increased after ET-1 infusion (400 pmol/kg). Monoclonal antibodies against ICAM-1 (1A29, 2 mg/kg), antineutrophil serum, and PAF antagonist (WEB-2086, 10 mg/kg) attenuated the mucosal barrier dysfunction induced by ET-1. Overall, our data indicate that ET-1 causes PMN accumulation, oxidant stress, and mucosal dysfunction in the rat small intestine and that ET-1-induced mucosal dysfunction involves a mechanism that includes a role for PMNs, ICAM-1, and PAF.

oxidant stress; mucosal permeability; intercellular adhesion molecule-1; platelet-activating factor


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Fujise, R. Iwakiri, B. Wu, S. Amemori, T. Kakimoto, F. Yokoyama, Y. Sakata, S. Tsunada, and K. Fujimoto
Apoptotic pathway in the rat small intestinal mucosa is different between fasting and ischemia-reperfusion
Am J Physiol Gastrointest Liver Physiol, July 1, 2006; 291(1): G110 - G116.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. E. King-VanVlack, J. D. Mewburn, C. K. Chapler, and P. H. MacDonald
Hemodynamic and proinflammatory actions of endothelin-1 in guinea pig small intestine submucosal microcirculation
Am J Physiol Gastrointest Liver Physiol, June 1, 2003; 284(6): G940 - G948.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Massai, P. Carbotti, C. Cambiaggi, M. Mencarelli, P. Migliaccio, M. Muscettola, and G. Grasso
Prepro-endothelin-1 mRNA and its mature peptide in human appendix
Am J Physiol Gastrointest Liver Physiol, February 1, 2003; 284(2): G340 - G348.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
B. K. Oktar, M. A. Gulpinar, A. Bozkurt, S. Ghandour, S. Cetinel, H. Moini, B. C. Yegen, S. Bilsel, D. N. Granger, and H. Kurtel
Endothelin receptor blockers reduce I/R-induced intestinal mucosal injury: role of blood flow
Am J Physiol Gastrointest Liver Physiol, April 1, 2002; 282(4): G647 - G655.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online