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Am J Physiol Gastrointest Liver Physiol 279: G733-G739, 2000;
0193-1857/00 $5.00
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Vol. 279, Issue 4, G733-G739, October 2000

Identification of gamma -aminobutyric acid receptor subunit types in human and rat liver

Ronit Erlitzki1, Yuewen Gong1, Manna Zhang1, and Gerald Minuk 1,2

Liver Diseases Unit, Departments of 1 Medicine and 2 Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Manitoba, Canada R3E 3P5

GABA is a potent inhibitory neurotransmitter that binds to heterooligomeric receptors in the mammalian brain. In a previous study, we documented specific GABA binding to isolated rat hepatocytes that resulted in inhibition of hepatocyte proliferation. The purpose of the present study was to define the nature of hepatic GABAA receptors and to document their expression during rapid liver growth (after partial hepatectomy). PCRs with gene-specific primers derived from published sequences were performed with Marathon-ready human and rat liver cDNA. Two GABAA receptor subunit types (beta 3 and epsilon ) were expressed in human liver and one subunit type (beta 3) in rat liver. PCR amplification of the human GABAA receptorbeta 3-subunit produced a single product (molecular mass 53-59 kDa). In the case of the epsilon -subunit, two PCR products were identified. After partial hepatectomy, GABAA receptorbeta 3-subunit expression inversely correlated with regenerative activity (r = -0.527, P = 0.006). In conclusion, these results indicate that in the human liver GABAA receptors consist of the beta 3- and epsilon -subunit types, whereas in the rat liver only the beta 3-subunit type is expressed. The results also support the hypothesis that GABAergic activity serves to maintain hepatocytes in a quiescent state.

gamma-aminobutyric acid A receptor; hepatocytes; receptors; neurotransmitters


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