AJP - GI AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 279: G775-G780, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (47)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hadjiagapiou, C.
Right arrow Articles by Ramaswamy, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hadjiagapiou, C.
Right arrow Articles by Ramaswamy, K.
Vol. 279, Issue 4, G775-G780, October 2000

Mechanism(s) of butyrate transport in Caco-2 cells: role of monocarboxylate transporter 1

Christos Hadjiagapiou, Larry Schmidt, Pradeep K. Dudeja, Thomas J. Layden, and Krishnamurthy Ramaswamy

Section of Digestive and Liver Diseases, Department of Medicine, University of Illinois at Chicago and the West Side Veterans Affairs Medical Center, Chicago, Illinois 60612

The short-chain fatty acid butyrate was readily taken up by Caco-2 cells. Transport exhibited saturation kinetics, was enhanced by low extracellular pH, and was Na+ independent. Butyrate uptake was unaffected by DIDS; however, alpha -cyano-4-hydroxycinnamate and the butyrate analogs propionate and L-lactate significantly inhibited uptake. These results suggest that butyrate transport by Caco-2 cells is mediated by a transporter belonging to the monocarboxylate transporter family. We identified five isoforms of this transporter, MCT1, MCT3, MCT4, MCT5, and MCT6, in Caco-2 cells by PCR, and MCT1 was found to be the most abundant isoform by RNase protection assay. Transient transfection of MCT1, in the antisense orientation, resulted in significant inhibition of butyrate uptake. The cells fully recovered from this inhibition by 5 days after transfection. In conclusion, our data showed that the MCT1 transporter may play a major role in the transport of butyrate into Caco-2 cells.

short-chain fatty acids; intestinal absorption; antisense cDNA


This article has been cited by other articles:


Home page
 Exp PhysiolHome page
D. T. Thwaites and C. M. H. Anderson
H+-coupled nutrient, micronutrient and drug transporters in the mammalian small intestine
Exp Physiol, July 1, 2007; 92(4): 603 - 619.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Graham, I. Gatherar, I. Haslam, M. Glanville, and N. L. Simmons
Expression and localization of monocarboxylate transporters and sodium/proton exchangers in bovine rumen epithelium
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R997 - R1007.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. Kirat, J. Masuoka, H. Hayashi, H. Iwano, H. Yokota, H. Taniyama, and S. Kato
Monocarboxylate transporter 1 (MCT1) plays a direct role in short-chain fatty acids absorption in caprine rumen
J. Physiol., October 15, 2006; 576(2): 635 - 647.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Borthakur, R. K. Gill, K. Hodges, K. Ramaswamy, G. Hecht, and P. K. Dudeja
Enteropathogenic Escherichia coli inhibits butyrate uptake in Caco-2 cells by altering the apical membrane MCT1 level
Am J Physiol Gastrointest Liver Physiol, January 1, 2006; 290(1): G30 - G35.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. K. Gill, S. Saksena, W. A. Alrefai, Z. Sarwar, J. L. Goldstein, R. E. Carroll, K. Ramaswamy, and P. K. Dudeja
Expression and membrane localization of MCT isoforms along the length of the human intestine
Am J Physiol Cell Physiol, October 1, 2005; 289(4): C846 - C852.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. Hadjiagapiou, A. Borthakur, R. Y. Dahdal, R. K. Gill, J. Malakooti, K. Ramaswamy, and P. K. Dudeja
Role of USF1 and USF2 as potential repressor proteins for human intestinal monocarboxylate transporter 1 promoter
Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1118 - G1126.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Vidyasagar, C. Barmeyer, J. Geibel, H. J. Binder, and V. M. Rajendran
Role of short-chain fatty acids in colonic HCO3 secretion
Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1217 - G1226.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
W. A. Alrefai, S. Tyagi, R. Gill, S. Saksena, C. Hadjiagapiou, F. Mansour, K. Ramaswamy, and P. K. Dudeja
Regulation of butyrate uptake in Caco-2 cells by phorbol 12-myristate 13-acetate
Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G197 - G203.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Tyagi, J. Venugopalakrishnan, K. Ramaswamy, and P. K. Dudeja
Mechanism of n-butyrate uptake in the human proximal colonic basolateral membranes
Am J Physiol Gastrointest Liver Physiol, April 1, 2002; 282(4): G676 - G682.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Lal, A. J. Kirkup, A. M. Brunsden, D. G. Thompson, and D. Grundy
Vagal afferent responses to fatty acids of different chain length in the rat
Am J Physiol Gastrointest Liver Physiol, October 1, 2001; 281(4): G907 - G915.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online