AJP - GI Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 279: G1023-G1030, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shah, V.
Right arrow Articles by Katusic, Z. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shah, V.
Right arrow Articles by Katusic, Z. S.
Vol. 279, Issue 5, G1023-G1030, November 2000

Gene transfer of recombinant endothelial nitric oxide synthase to liver in vivo and in vitro

Vijay Shah, Alex F. Chen, Sheng Cao, Helen Hendrickson, Deb Weiler, Leslie Smith, Janet Yao, and Zvonimir S. Katusic

Gastrointestinal Research Unit and Anesthesia Research Unit, Mayo Clinic, Rochester, Minnesota 55905

Endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) contributes to hepatic vascular homeostasis. The aim of this study was to examine whether delivery of an adenoviral vector encoding eNOS gene to liver affects vasomotor function in vivo and the mechanism of NO production in vitro. Rats were administered adenoviruses encoding beta -galactosidase (AdCMVLacZ) or eNOS (AdCMVeNOS) via tail vein injection and studied 1 wk later. In animals transduced with AdCMVLacZ, beta -galactosidase activity was increased in the liver, most prominently in hepatocytes. In AdCMVeNOS-transduced animals, eNOS protein levels and catalytic activity were significantly increased. Overexpression of eNOS diminished baseline perfusion pressure and constriction in response to the alpha 1-agonist methoxamine in the perfused liver. Transduction of cultured hepatocytes with AdCMVeNOS resulted in the targeting of recombinant eNOS to a perinuclear distribution and binding with the NOS-activating protein heat shock protein 90. These events were associated with increased ionomycin-stimulated NO release. In summary, this is the first study to demonstrate successful delivery of the recombinant eNOS gene to liver in vivo and in vitro with ensuing NO production.

hepatic perfusion; adenovirus vector; beta -galactosidase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H. Hendrickson, S. Chatterjee, S. Cao, M. M. Ruiz, W. C. Sessa, and V. Shah
Influence of caveolin on constitutively activated recombinant eNOS: insights into eNOS dysfunction in BDL rat liver
Am J Physiol Gastrointest Liver Physiol, August 8, 2003; 285(3): G652 - G660.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
M Van de Casteele, A Omasta, S Janssens, T Roskams, V Desmet, F Nevens, and J Fevery
In vivo gene transfer of endothelial nitric oxide synthase decreases portal pressure in anaesthetised carbon tetrachloride cirrhotic rats
Gut, September 1, 2002; 51(3): 440 - 445.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Iwakiri, M.-H. Tsai, T. J. McCabe, J.-P. Gratton, D. Fulton, R. J. Groszmann, and W. C. Sessa
Phosphorylation of eNOS initiates excessive NO production in early phases of portal hypertension
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2084 - H2090.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
V. Shah, S. Cao, H. Hendrickson, J. Yao, and Z. S. Katusic
Regulation of hepatic eNOS by caveolin and calmodulin after bile duct ligation in rats
Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1209 - G1216.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Iwakiri, M.-H. Tsai, T. J. McCabe, J.-P. Gratton, D. Fulton, R. J. Groszmann, and W. C. Sessa
Phosphorylation of eNOS initiates excessive NO production in early phases of portal hypertension
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2084 - H2090.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online