| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Laboratory of Pediatric Gastroenterology and Nutrition, Erasmus University and Sophia Children's Hospital, 3015GE Rotterdam; and 2 Department of Pediatrics, Academic Medical Center, 1105AZ Amsterdam, The Netherlands
Proliferation, differentiation, and cell death were studied in small intestinal and colonic epithelia of rats after treatment with methotrexate. Days 1-2 after treatment were characterized by decreased proliferation, increased apoptosis, and decreased numbers and depths of small intestinal crypts in a proximal-to-distal decreasing gradient along the small intestine. The remaining crypt epithelium appeared flattened, except for Paneth cells, in which lysozyme protein and mRNA expression was increased. Regeneration through increased proliferation during days 3-4 coincided with villus atrophy, showing decreased numbers of villus enterocytes and decreased expression of the enterocyte-specific genes sucrase-isomaltase and carbamoyl phosphate synthase I. Remarkably, goblet cells were spared at villus tips and remained functional, displaying Muc2 and trefoil factor 3 expression. On days 8-10, all parameters had returned to normal in the whole small intestine. No methotrexate-induced changes were seen in epithelial morphology, proliferation, apoptosis, Muc2, and TFF3 immunostaining in the colon. The observed small intestinal sparing of Paneth cells and goblet cells following exposure to methotrexate is likely to contribute to epithelial defense during increased vulnerability of the intestinal epithelium.
villus atrophy; chemotherapy; gene expression; damage and regeneration
This article has been cited by other articles:
|
|
 |
|
  A. M. Stringer, R. J. Gibson, R. M. Logan, J. M. Bowen, A. S. J. Yeoh, J. Hamilton, and D. M. K. Keefe Gastrointestinal Microflora and Mucins May Play a Critical Role in the Development of 5-Fluorouracil-Induced Gastrointestinal Mucositis Experimental Biology and Medicine, April 1, 2009; 234(4): 430 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. M. Derikx, N. M. A. Blijlevens, J. P. Donnelly, H. Fujii, T. Kanda, A. A. van Bijnen, E. Heineman, and W. A. Buurman Loss of enterocyte mass is accompanied by diminished turnover of enterocytes after myeloablative therapy in haematopoietic stem-cell transplant recipients Ann. Onc., February 1, 2009; 20(2): 337 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. J. Reimerink, J. A. Boshuizen, A. W. C. Einerhand, E. Duizer, G. van Amerongen, N. Schmidt, and M. P. G. Koopmans Systemic immune response after rotavirus inoculation of neonatal mice depends on source and level of purification of the virus: implications for the use of heterologous vaccine candidates J. Gen. Virol., February 1, 2007; 88(2): 604 - 612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. de Koning, J. M van Dieren, D. J Lindenbergh-Kortleve, M. van der Sluis, T. Matsumoto, K. Yamaguchi, A. W Einerhand, J. N Samsom, R. Pieters, and E. E. Nieuwenhuis Contributions of mucosal immune cells to methotrexate-induced mucositis Int. Immunol., June 1, 2006; 18(6): 941 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Horie, T. Li, K. Ito, S.-i. Sumi, and T. Fuwa Aged Garlic Extract Protects against Methotrexate-Induced Apoptotic Cell Injury of IEC-6 Cells J. Nutr., March 1, 2006; 136(3): 861S - 863S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Li, K Ito, S-i Sumi, T Fuwa, and T Horie Antiapoptosis action of aged garlic extract (AGE) protects epithelial cells from methotrexate induced injury Gut, December 1, 2005; 54(12): 1819 - 1820. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kim, A. J. Schein, and J. A. Nadel E-cadherin promotes EGFR-mediated cell differentiation and MUC5AC mucin expression in cultured human airway epithelial cells Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1049 - L1060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Boshuizen, J. H. J. Reimerink, A. M. Korteland-van Male, V. J. J. van Ham, M. P. G. Koopmans, H. A. Buller, J. Dekker, and A. W. C. Einerhand Changes in Small Intestinal Homeostasis, Morphology, and Gene Expression during Rotavirus Infection of Infant Mice J. Virol., December 15, 2003; 77(24): 13005 - 13016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Conour, D. Ganessunker, K. A. Tappenden, S. M. Donovan, and H. R. Gaskins Acidomucin goblet cell expansion induced by parenteral nutrition in the small intestine of piglets Am J Physiol Gastrointest Liver Physiol, November 1, 2002; 283(5): G1185 - G1196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. B. Renes, M. Verburg, D. J. P. M. Van Nispen, H. A. Buller, J. Dekker, and A. W. C. Einerhand Distinct epithelial responses in experimental colitis: implications for ion uptake and mucosal protection Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G169 - G179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. B. Renes, J. A. Boshuizen, D. J. P. M. Van Nispen, N. P. Bulsing, H. A. Buller, J. Dekker, and A. W. C. Einerhand Alterations in Muc2 biosynthesis and secretion during dextran sulfate sodium-induced colitis Am J Physiol Gastrointest Liver Physiol, February 1, 2002; 282(2): G382 - G389. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
