AJP - GI Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 279: G893-G902, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wada-Takahashi, S.
Right arrow Articles by Tamura, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wada-Takahashi, S.
Right arrow Articles by Tamura, K.
Vol. 279, Issue 5, G893-G902, November 2000

Actions of reactive oxygen species on AH/type 2 myenteric neurons in guinea pig distal colon

S. Wada-Takahashi and K. Tamura

Department of Physiology, Kanagawa Dental College, Yokosuka 238-8580, Japan

With conventional intracellular recording methods, we investigated the mechanism of actions of reactive oxygen species (ROS) derived from hypoxanthine and xanthine oxidase (HX/XO) reactions on AH/type 2 myenteric neurons in the guinea pig distal colon. Of the 54 neurons to which HX/XO was applied, 32 neurons showed a transient membrane hyperpolarization(s) followed by a long-lasting membrane depolarization. Two additional groups of 10 myenteric neurons exhibited only a membrane hyperpolarization(s) or a late-onset membrane depolarization, respectively, and the remaining two neurons did not show any response to HX/XO. Analysis of changes of the input resistance induced by HX/XO indicated that suppression and augmentation of the conductance of Ca2+-dependent K+ channels are the ionic mechanisms underlying the membrane hyperpolarization and depolarization, respectively. The effects of HX/XO on myenteric neurons were mimicked by application of caffeine or H2O2. The results suggest that OH·, but neither H2O2 nor O2·-, is responsible for HX/XO-induced responses. The intracellular Ca2+ store may be the acting site of ROS in colonic AH/type 2 neurons.

superoxide anion radical; hydrogen peroxide; hydroxyl radical; AH/type 2 neuron


This article has been cited by other articles:


Home page
 J. Physiol.Home page
E. M. Krauter, D. R. Linden, K. A. Sharkey, and G. M. Mawe
Synaptic plasticity in myenteric neurons of the guinea-pig distal colon: presynaptic mechanisms of inflammation-induced synaptic facilitation
J. Physiol., June 1, 2007; 581(2): 787 - 800.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
T. Ruan, Y. S. Lin, K.-S. Lin, and Y. R. Kou
Sensory transduction of pulmonary reactive oxygen species by capsaicin-sensitive vagal lung afferent fibres in rats
J. Physiol., June 1, 2005; 565(2): 563 - 578.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. R. Linden, K. A. Sharkey, W. Ho, and G. M. Mawe
Cyclooxygenase-2 contributes to dysmotility and enhanced excitability of myenteric AH neurones in the inflamed guinea pig distal colon
J. Physiol., May 15, 2004; 557(1): 191 - 205.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
F. Vogalis and J. R. Harvey
Altered Excitability of Intestinal Neurons in Primary Culture Caused by Acute Oxidative Stress
J Neurophysiol, June 1, 2003; 89(6): 3039 - 3050.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Ruan, C.-Y. Ho, and Y. R. Kou
Afferent vagal pathways mediating respiratory reflexes evoked by ROS in the lungs of anesthetized rats
J Appl Physiol, May 1, 2003; 94(5): 1987 - 1998.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. Vanden Berghe, J. L. Kenyon, and T. K. Smith
Mitochondrial Ca2+ Uptake Regulates the Excitability of Myenteric Neurons
J. Neurosci., August 15, 2002; 22(16): 6962 - 6971.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online