| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Internal Medicine and 2 Pathology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157
The rat and mouse organic anion-transporting polypeptides (oatp) subtype 3 (oatp3) were cloned to further define components of the intestinal bile acid transport system. In transfected COS cells, oatp3 mediated Na+-independent, DIDS-inhibited taurocholate uptake (Michaelis-Menten constant ~30 µM). The oatp3-mediated uptake rates and affinities were highest for glycine-conjugated dihydroxy bile acids. In stably transfected, polarized Madin-Darby canine kidney (MDCK) cells, oatp3 mediated only apical uptake of taurocholate. RT-PCR analysis revealed that rat oatp3, but not oatp1 or oatp2, was expressed in small intestine. By RNase protection assay, oatp3 mRNA was readily detected down the length of the small intestine as well as in brain, lung, and retina. An antibody directed to the carboxy terminus localized oatp3 to the apical brush-border membrane of rat jejunal enterocytes. The mouse oatp3 gene was localized to a region of mouse chromosome 6. This region is syntenic with human chromosome 12p12, where the human OATP-A gene was mapped, suggesting that rodent oatp3 is orthologous to the human OATP-A. These transport and expression properties suggest that rat oatp3 mediates the anion exchange-driven absorption of bile acids previously described for the proximal small intestine.
intestinal transport; brush-border membranes; organic anion transport; taurocholate
This article has been cited by other articles:
|
|
 |
|
  C. Mahagita, S. M. Grassl, P. Piyachaturawat, and N. Ballatori Human organic anion transporter 1B1 and 1B3 function as bidirectional carriers and do not mediate GSH-bile acid cotransport Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G271 - G278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Sehayek, L. R. Hagey, Y.-Y. Fung, E. M. Duncan, H. J. Yu, G. Eggertsen, I. Bjorkhem, A. F. Hofmann, and J. L. Breslow Two loci on chromosome 9 control bile acid composition: evidence that a strong candidate gene, Cyp8b1, is not the culprit J. Lipid Res., September 1, 2006; 47(9): 2020 - 2027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kis, T. Isse, J. A. Snipes, L. Chen, H. Yamashita, Y. Ueta, and D. W. Busija Effects of LPS stimulation on the expression of prostaglandin carriers in the cells of the blood-brain and blood-cerebrospinal fluid barriers J Appl Physiol, April 1, 2006; 100(4): 1392 - 1399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Weihrauch, J. Kanchanapoo, M. Ao, R. Prasad, P. Piyachaturawat, and M. C. Rao Weanling, but not adult, rabbit colon absorbs bile acids: flux is linked to expression of putative bile acid transporters Am J Physiol Gastrointest Liver Physiol, March 1, 2006; 290(3): G439 - G450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. St-Pierre, T. Stallmach, A. Freimoser Grundschober, J.-F. Dufour, M. A. Serrano, J. J. G. Marin, Y. Sugiyama, and P. J. Meier Temporal expression profiles of organic anion transport proteins in placenta and fetal liver of the rat Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2004; 287(6): R1505 - R1516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shoji, H. Suzuki, H. Kusuhara, Y. Watanabe, S. Sakamoto, and Y. Sugiyama ATP-dependent transport of organic anions into isolated basolateral membrane vesicles from rat intestine Am J Physiol Gastrointest Liver Physiol, October 1, 2004; 287(4): G749 - G756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Dawson, J. Haywood, A. L. Craddock, M. Wilson, M. Tietjen, K. Kluckman, N. Maeda, and J. S. Parks Targeted Deletion of the Ileal Bile Acid Transporter Eliminates Enterohepatic Cycling of Bile Acids in Mice J. Biol. Chem., September 5, 2003; 278(36): 33920 - 33927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Mizuno, T. Niwa, Y. Yotsumoto, and Y. Sugiyama Impact of Drug Transporter Studies on Drug Discovery and Development Pharmacol. Rev., September 1, 2003; 55(3): 425 - 461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Trauner and J. L. Boyer Bile Salt Transporters: Molecular Characterization, Function, and Regulation Physiol Rev, April 1, 2003; 83(2): 633 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Sweet, L. M. S. Chan, R. Walden, X.-P. Yang, D. S. Miller, and J. B. Pritchard Organic anion transporter 3 (Slc22a8) is a dicarboxylate exchanger indirectly coupled to the Na+ gradient Am J Physiol Renal Physiol, April 1, 2003; 284(4): F763 - F769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Leazer and C. D. Klaassen The Presence of Xenobiotic Transporters in Rat Placenta Drug Metab. Dispos., February 1, 2003; 31(2): 153 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Gotoh, Y. Kato, B. Stieger, P. J. Meier, and Y. Sugiyama Gender difference in the Oatp1-mediated tubular reabsorption of estradiol 17beta -D-glucuronide in rats Am J Physiol Endocrinol Metab, June 1, 2002; 282(6): E1245 - E1254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Li, D. P. Hartley, N. J. Cherrington, and C. D. Klaassen Tissue Expression, Ontogeny, and Inducibility of Rat Organic Anion Transporting Polypeptide 4 J. Pharmacol. Exp. Ther., May 1, 2002; 301(2): 551 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-Y. Cai, W. Wang, C. J. Soroka, N. Ballatori, and J. L. Boyer An evolutionarily ancient Oatp: insights into conserved functional domains of these proteins Am J Physiol Gastrointest Liver Physiol, April 1, 2002; 282(4): G702 - G710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cong, A. K. Y. Fong, R. Lee, and K. S. Pang Absorption of Benzoic Acid in Segmental Regions of the Vascularly Perfused Rat Small Intestine Preparation Drug Metab. Dispos., December 1, 2001; 29(12): 1539 - 1547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Gui, P. R. Dobner, and R. E. Carraway Endogenous neurotensin facilitates enterohepatic bile acid circulation by enhancing intestinal uptake in rats Am J Physiol Gastrointest Liver Physiol, December 1, 2001; 281(6): G1413 - G1422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Chen, L. Ma, N. Al-Ansari, and B. Shneider The Role of AP-1 in the Transcriptional Regulation of the Rat Apical Sodium-dependent Bile Acid Transporter J. Biol. Chem., October 12, 2001; 276(42): 38703 - 38714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Mottino, T. Hoffman, P. A. Dawson, M. G. Luquita, J. A. Monti, E. J. Sanchez Pozzi, V. A. Catania, J. Cao, and M. Vore Increased expression of ileal apical sodium-dependent bile acid transporter in postpartum rats Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G41 - G50. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
