| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 United States Department of Agriculture, Agricultural Research Service, Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030; 2 Departments of Animal Science and Health, Royal Veterinary and Agricultural University, DK-1870 Copenhagen, Denmark; 3 Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi 39762; and 4 Department of Medical Physiology, University of Copenhagen, DK-2200 Copenhagen, Denmark
We wished to
determine whether exogenous glucagon-like peptide (GLP)-2 infusion
stimulates intestinal growth in parenterally fed immature pigs. Piglets
(106-108 days gestation) were given parenteral nutrient infusion
(TPN), TPN + human GLP-2 (25 nmol · kg
1 · day1), or
sow's milk enterally (ENT) for 6 days. Intestinal protein synthesis
was then measured in vivo after a bolus dose of
[1-13C]phenylalanine, and degradation was calculated from
the difference between protein accretion and synthesis. Crypt cell
proliferation and apoptosis were measured in situ by
5-bromodeoxyuridine (BrdU) and terminal dUTP nick-end labeling (TUNEL),
respectively. Intestinal protein and DNA accretion rates and villus
heights were similar in GLP-2 and ENT pigs, and both were higher
(P < 0.05) than in TPN pigs. GLP-2 decreased
fractional protein degradation rate, whereas ENT increased fractional
protein synthesis rate compared with TPN pigs. Percentage of
TUNEL-positive cells in GLP-2 and ENT groups was 48 and 64% lower,
respectively, than in TPN group (P < 0.05). However,
ENT, but not GLP-2, increased percentage of BrdU-positive crypt cells
above that in TPN piglets. We conclude that GLP-2 increases intestinal
growth in premature, TPN-fed pigs by decreasing proteolysis and
apoptosis, whereas enteral nutrition acts via increased protein
synthesis and cell proliferation and decreased apoptosis.
protein synthesis; protein degradation; total parenteral nutrition; preterm infants
This article has been cited by other articles:
|
|
 |
|
  H. Amin, J. J. Holst, B. Hartmann, L. Wallace, J. Wright, and D. L. Sigalet Functional Ontogeny of the Proglucagon-Derived Peptide Axis in the Premature Human Neonate Pediatrics, January 1, 2008; 121(1): e180 - e186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. T. Sangild, C. Malo, M. Schmidt, Y. M. Petersen, J. Elnif, J. J. Holst, and R. K. Buddington Glucagon-like peptide 2 has limited efficacy to increase nutrient absorption in fetal and preterm pigs Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2007; 293(6): R2179 - R2184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Dube and P. L. Brubaker Frontiers in glucagon-like peptide-2: multiple actions, multiple mediators Am J Physiol Endocrinol Metab, August 1, 2007; 293(2): E460 - E465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Simonsen, S. Pilgaard, C. Orskov, M. M. Rosenkilde, B. Hartmann, J. J. Holst, and C. F. Deacon Exendin-4, but not dipeptidyl peptidase IV inhibition, increases small intestinal mass in GK rats Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G288 - G295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Commare and K. A. Tappenden Development of the Infant Intestine: Implications for Nutrition Support Nutr Clin Pract, April 1, 2007; 22(2): 159 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Kien, R. Blauwiekel, J. Y. Bunn, T. L. Jetton, W. L. Frankel, and J. J. Holst Cecal Infusion of Butyrate Increases Intestinal Cell Proliferation in Piglets J. Nutr., April 1, 2007; 137(4): 916 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Urschel, A. R. Evans, C. W. Wilkinson, P. B. Pencharz, and R. O. Ball Parenterally Fed Neonatal Piglets Have a Low Rate of Endogenous Arginine Synthesis from Circulating Proline J. Nutr., March 1, 2007; 137(3): 601 - 606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Burrin, B. Stoll, X. Guan, L. Cui, X. Chang, and D. Hadsell GLP-2 rapidly activates divergent intracellular signaling pathways involved in intestinal cell survival and proliferation in neonatal piglets Am J Physiol Endocrinol Metab, January 1, 2007; 292(1): E281 - E291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. T. Sangild Gut Responses to Enteral Nutrition in Preterm Infants and Animals Experimental Biology and Medicine, December 1, 2006; 231(11): 1695 - 1711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Liu, D. W Nelson, J. J Holst, and D. M Ney Synergistic effect of supplemental enteral nutrients and exogenous glucagon-like peptide 2 on intestinal adaptation in a rat model of short bowel syndrome. Am. J. Clinical Nutrition, November 1, 2006; 84(5): 1142 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Sun, A. U. Spencer, H. Yang, E. Q. Haxhija, and D. H. Teitelbaum Impact of Caloric Intake on Parenteral Nutrition-Associated Intestinal Morphology and Mucosal Barrier Function JPEN J Parenter Enteral Nutr, November 1, 2006; 30(6): 474 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. BRUBAKER The Glucagon-Like Peptides: Pleiotropic Regulators of Nutrient Homeostasis Ann. N.Y. Acad. Sci., July 1, 2006; 1070(1): 10 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Cottrell, B. Stoll, R. K. Buddington, J. E. Stephens, L. Cui, X. Chang, and D. G. Burrin Glucagon-like peptide-2 protects against TPN-induced intestinal hexose malabsorption in enterally refed piglets Am J Physiol Gastrointest Liver Physiol, February 1, 2006; 290(2): G293 - G300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M Sinclair and D. J. Drucker Proglucagon-Derived Peptides: Mechanisms of Action and Therapeutic Potential Physiology, October 1, 2005; 20(5): 357 - 365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Iordache, L. Drozdowski, M. T. Clandinin, G. Wild, Z. Todd, and A. B. R. Thomson Treatment of suckling rats with GLP-2 plus dexamethasone increases the ileal uptake of fatty acids in later life Am J Physiol Gastrointest Liver Physiol, January 1, 2005; 288(1): G54 - G59. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Martin, L. E. Wallace, and D. L. Sigalet Glucagon-like peptide-2 induces intestinal adaptation in parenterally fed rats with short bowel syndrome Am J Physiol Gastrointest Liver Physiol, June 1, 2004; 286(6): G964 - G972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kansagra, B. Stoll, C. Rognerud, H. Niinikoski, C.-N. Ou, R. Harvey, and D. Burrin Total parenteral nutrition adversely affects gut barrier function in neonatal piglets Am J Physiol Gastrointest Liver Physiol, December 1, 2003; 285(6): G1162 - G1170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Estall and D. J. Drucker Dual Regulation of Cell Proliferation and Survival via Activation of Glucagon-Like Peptide-2 Receptor Signaling J. Nutr., November 1, 2003; 133(11): 3708 - 3711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Tappenden, D. M. Albin, A. L. Bartholome, and H. F. Mangian Glucagon-Like Peptide-2 and Short-Chain Fatty Acids: A New Twist to an Old Story J. Nutr., November 1, 2003; 133(11): 3717 - 3720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G Burrin, B. Stoll, X. Chang, J. B van Goudoever, H. Fujii, S. M Hutson, and P. J Reeds Parenteral nutrition results in impaired lactose digestion and hexose absorption when enteral feeding is initiated in infant pigs Am. J. Clinical Nutrition, September 1, 2003; 78(3): 461 - 470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. J. Adegoke, M. I. McBurney, S. E. Samuels, and V. E. Baracos Modulation of intestinal protein synthesis and protease mRNA by luminal and systemic nutrients Am J Physiol Gastrointest Liver Physiol, June 1, 2003; 284(6): G1017 - G1026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yang, Y. Fan, and D. H. Teitelbaum Intraepithelial lymphocyte-derived interferon-gamma evokes enterocyte apoptosis with parenteral nutrition in mice Am J Physiol Gastrointest Liver Physiol, April 1, 2003; 284(4): G629 - G637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Dahly, M. B. Gillingham, Z. Guo, S. G. Murali, D. W. Nelson, J. J. Holst, and D. M. Ney Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection Am J Physiol Gastrointest Liver Physiol, April 1, 2003; 284(4): G670 - G682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. T. Sangild, Y. M. Petersen, M. Schmidt, J. Elnif, T. K. Petersen, R. K. Buddington, G. Greisen, K. F. Michaelsen, and D. G. Burrin Preterm Birth Affects the Intestinal Response to Parenteral and Enteral Nutrition in Newborn Pigs J. Nutr., September 1, 2002; 132(9): 2673 - 2681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yusta, J. Estall, and D. J. Drucker Glucagon-like Peptide-2 Receptor Activation Engages Bad and Glycogen Synthase Kinase-3 in a Protein Kinase A-dependent Manner and Prevents Apoptosis following Inhibition of Phosphatidylinositol 3-Kinase J. Biol. Chem., July 5, 2002; 277(28): 24896 - 24906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Dahly, Z. Guo, and D. M. Ney Alterations in Enterocyte Proliferation and Apoptosis Accompany TPN-Induced Mucosal Hypoplasia and IGF-I-Induced Hyperplasia in Rats J. Nutr., July 1, 2002; 132(7): 2010 - 2014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D J Drucker Gut adaptation and the glucagon-like peptides Gut, March 1, 2002; 50(3): 428 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Petersen, D. G. Burrin, and P. T. Sangild GLP-2 has differential effects on small intestine growth and function in fetal and neonatal pigs Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2001; 281(6): R1986 - R1993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. van Goudoever, B. Stoll, B. Hartmann, J. J. Holst, P. J. Reeds, and D. G. Burrin Secretion of Trophic Gut Peptides Is Not Different in Bolus- and Continuously Fed Piglets J. Nutr., March 1, 2001; 131(3): 729 - 732. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
