AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 279: G1307-G1322, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (42)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pucilowska, J. B.
Right arrow Articles by Lund, P. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pucilowska, J. B.
Right arrow Articles by Lund, P. K.
Vol. 279, Issue 6, G1307-G1322, December 2000

IGF-I and procollagen alpha 1(I) are coexpressed in a subset of mesenchymal cells in active Crohn's disease

Jolanta B. Pucilowska1,2, Kirk K. McNaughton1, Nirupama K. Mohapatra1,2, Eileen C. Hoyt1,2, Ellen M. Zimmermann3, R. Balfour Sartor2,4, and P. Kay Lund1,2

1 Department of Cell and Molecular Physiology, 2 Center for Gastrointestinal Biology and Disease, and 4 Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7545; and 3 Department of Medicine, University of Michigan, Ann Arbor, Michigan 48109-0586

This study tested the hypothesis that insulin-like growth factor I (IGF-I) expression is increased at sites of fibrosis in diseased intestine of patients with Crohn's disease (CD). IGF-I mRNA was quantified by RNase protection assay in uninvolved and involved intestine of 13 CD patients (10 ileum, 3 colon) and 7 ulcerative colitis (UC) patients (colon). In situ hybridization histochemistry compared the localization of IGF-I and procollagen alpha 1(I) mRNAs. Masson's trichrome staining and immunohistochemistry for IGF-I precursor, alpha -smooth muscle actin (A), vimentin (V), desmin (D), and c-kit were used to examine the mesenchymal cell subtypes that express IGF-I and collagen in uninvolved and involved ileum and colon of CD patients and "normal" ileum and colon from noninflammatory controls. IGF-I mRNA was elevated in involved ileum and colon of patients with CD but not in involved colon of patients with UC. IGF-I and procollagen alpha 1(I) mRNA showed overlapping distribution within fibrotic submucosa and muscularis propria of involved CD ileum and colon. In involved CD intestine, increased IGF-I precursor expression localized to mesenchymal cells in regions of tissue disorganization and fibrosis in muscularis mucosa, submucosa, and muscularis propria. In these regions, there were increased numbers of V+ cells relative to normal or uninvolved intestine. Increased IGF-I expression was localized to cells with a phenotype typical of fibroblasts (V+/A-/D-), myofibroblasts (V+/A+/D+), and, to a lesser extent, cells with normal enteric smooth muscle phenotype (V-/A+/D+). We conclude that increased IGF-I expression in multiple mesenchymal cell subtypes and increased numbers of cells with fibroblast/myofibroblast phenotype are involved in fibrosis associated with CD.

intestinal fibrosis; mesenchymal cells


This article has been cited by other articles:


Home page
 GutHome page
F Rieder, J Brenmoehl, S Leeb, J Scholmerich, and G Rogler
Wound healing and fibrosis in intestinal disease
Gut, January 1, 2007; 56(1): 130 - 139.
[Full Text] [PDF]

Home page
 GutHome page
Y Motomura, W I Khan, R T El-Sharkawy, M Verma-Gandhu, E F Verdu, J Gauldie, and S M Collins
Induction of a fibrogenic response in mouse colon by overexpression of monocyte chemoattractant protein 1
Gut, May 1, 2006; 55(5): 662 - 670.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Fruchtman, J. G. Simmons, C. Z. Michaylira, M. E. Miller, C. J. Greenhalgh, D. M. Ney, and P. K. Lund
Suppressor of cytokine signaling-2 modulates the fibrogenic actions of GH and IGF-I in intestinal mesenchymal cells
Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G342 - G350.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
B. A. Vallance, M. I. Gunawan, B. Hewlett, P. Bercik, C. Van Kampen, F. Galeazzi, P. J. Sime, J. Gauldie, and S. M. Collins
TGF-{beta}1 gene transfer to the mouse colon leads to intestinal fibrosis
Am J Physiol Gastrointest Liver Physiol, July 1, 2005; 289(1): G116 - G128.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Chitapanarux, S. L. Chen, H. Lee, A. C. Melton, and H. F. Yee Jr.
C-type natriuretic peptide induces human colonic myofibroblast relaxation
Am J Physiol Gastrointest Liver Physiol, January 1, 2004; 286(1): G31 - G36.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
K. L. Williams, C. R. Fuller, J. Fagin, and P. K. Lund
Mesenchymal IGF-I overexpression: paracrine effects in the intestine, distinct from endocrine actions
Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G875 - G885.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. G. Simmons, J. B. Pucilowska, T. O. Keku, and P. K. Lund
IGF-I and TGF-beta 1 have distinct effects on phenotype and proliferation of intestinal fibroblasts
Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G809 - G818.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online