AJP - GI Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 279: G1343-G1354, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Toivola, D. M.
Right arrow Articles by Omary, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Toivola, D. M.
Right arrow Articles by Omary, M. B.
Vol. 279, Issue 6, G1343-G1354, December 2000

Simple epithelial keratins are dispensable for cytoprotection in two pancreatitis models

Diana M. Toivola1, Hélène Baribault2, Thomas Magin3, Sara A. Michie4, and M. Bishr Omary1

Departments of 1 Medicine and 4 Pathology, Palo Alto Veterans Affairs Medical Center and Stanford University, Palo Alto 94304; 2 Deltagen, San Carlos, California 94205; and 3 University of Bonn, D-53117 Bonn, Germany

Pancreatic acinar cells express keratins 8 and 18 (K8/18), which form cytoplasmic filament (CF) and apicolateral filament (ALF) pools. Hepatocyte K8/18 CF provide important protection from environmental stresses, but disruption of acinar cell CF has no significant impact. We asked whether acinar cell ALF are important in providing cytoprotective roles by studying keratin filaments in pancreata of K8- and K18-null mice. K8-null pancreas lacks both keratin pools, but K18-null pancreas lacks only CF. Mouse but not human acinar cells also express apicolateral keratin 19 (K19), which explains the presence of apicolateral keratins in K18-null pancreas. K8- and K18-null pancreata are histologically normal, and their acini respond similarly to stimulated secretion, although K8-null acini viability is reduced. Absence of total filaments (K8-null) or CF (K18-null) does not increase susceptibility to pancreatitis induced by caerulein or a choline-deficient diet. In normal and K18-null acini, K19 is upregulated after caerulein injury and, unexpectedly, forms CF. As in hepatocytes, acinar injury is also associated with keratin hyperphosphorylation. Hence, K19 forms ALF in mouse acinar cells and helps define two distinct ALF and CF pools. On injury, K19 forms CF that revert to ALF after healing. Acinar keratins appear to be dispensable for cytoprotection, in contrast to hepatocyte keratins, despite similar hyperphosphorylation patterns after injury.

acute pancreatitis; acinar cell; caerulein; choline-deficient diet; keratin phosphorylation


This article has been cited by other articles:


Home page
 JCBHome page
D. M. Toivola, S. Krishnan, H. J. Binder, S. K. Singh, and M. B. Omary
Keratins modulate colonocyte electrolyte transport via protein mistargeting
J. Cell Biol., March 15, 2004; 164(6): 911 - 921.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
G.-Z. Tao, D. M. Toivola, B. Zhong, S. A. Michie, E. Z. Resurreccion, Y. Tamai, M. M. Taketo, and M. B. Omary
Keratin-8 null mice have different gallbladder and liver susceptibility to lithogenic diet-induced injury
J. Cell Sci., November 15, 2003; 116(22): 4629 - 4638.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
P. Wong and P. A. Coulombe
Loss of keratin 6 (K6) proteins reveals a function for intermediate filaments during wound repair
J. Cell Biol., October 27, 2003; 163(2): 327 - 337.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N.-O. Ku, S. Michie, E. Z. Resurreccion, R. L. Broome, and M. B. Omary
Keratin binding to 14-3-3 proteins modulates keratin filaments and hepatocyte mitotic progression
PNAS, April 2, 2002; 99(7): 4373 - 4378.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online