| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
2 Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, and 1 Graduate School of the Biomedical Sciences, Newark, New Jersey 07103-2714
Fructose transporter (GLUT-5) expression is low in mid-weaning rat small intestine, increases normally after weaning is completed, and can be precociously induced by premature consumption of a high-fructose (HF) diet. In this study, an in vivo perfusion model was used to determine the mechanisms regulating this substrate-induced reprogramming of GLUT-5 development. HF (100 mM) but not high-glucose (HG) perfusion increased GLUT-5 activity and mRNA abundance. In contrast, HF and HG perfusion had no effect on Na+-dependent glucose transporter (SGLT-1) expression but increased c-fos and c-jun expression. Intraperitoneal injection of actinomycin D before intestinal perfusion blocked the HF-induced increase in fructose uptake rate and GLUT-5 mRNA abundance. Actinomycin D also prevented the perfusion-induced increase in c-fos and c-jun mRNA abundance but did not affect glucose uptake rate and SGLT-1 mRNA abundance. Cycloheximide blocked the HF-induced increase in fructose uptake rate but not the increase in GLUT-5 mRNA abundance and had no effect on glucose uptake rate and SGLT-1 mRNA abundance. In neonatal rats, the substrate-induced reprogramming of intestinal fructose transport is likely to involve transcription and translation of the GLUT-5 gene.
membranes; mucosa; nutrient transport; sodium ion-dependent glucose transporter; sugars
This article has been cited by other articles:
|
|
 |
|
  V. Douard and R. P. Ferraris Regulation of the fructose transporter GLUT5 in health and disease Am J Physiol Endocrinol Metab, August 1, 2008; 295(2): E227 - E237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Douard, H.-I. Choi, S. Elshenawy, D. Lagunoff, and R. P. Ferraris Developmental reprogramming of rat GLUT5 requires glucocorticoid receptor translocation to the nucleus J. Physiol., August 1, 2008; 586(15): 3657 - 3673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kirchner, A. Muduli, D. Casirola, K. Prum, V. Douard, and R. P Ferraris Luminal fructose inhibits rat intestinal sodium-phosphate cotransporter gene expression and phosphate uptake Am. J. Clinical Nutrition, April 1, 2008; 87(4): 1028 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kirchner, E. Kwon, A. Muduli, C. Cerqueira, X.-L. Cui, and R. P. Ferraris Vanadate but Not Tungstate Prevents the Fructose-Induced Increase in GLUT5 Expression and Fructose Uptake by Neonatal Rat Intestine J. Nutr., September 1, 2006; 136(9): 2308 - 2313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-L. Cui, A. M. Schlesier, E. L. Fisher, C. Cerqueira, and R. P. Ferraris Fructose-induced increases in neonatal rat intestinal fructose transport involve the PI3-kinase/Akt signaling pathway Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1310 - G1320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-L. Cui, P. Soteropoulos, P. Tolias, and R. P. Ferraris Fructose-responsive genes in the small intestine of neonatal rats Physiol Genomics, July 8, 2004; 18(2): 206 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Jiang, H. Lawsky, R. M. Coloso, M. A. Dudley, and R. P. Ferraris Intestinal perfusion induces rapid activation of immediate-early genes in weaning rats Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2001; 281(4): R1274 - R1282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Jiang, E. S. David, N. Espina, and R. P. Ferraris GLUT-5 expression in neonatal rats: crypt-villus location and age-dependent regulation Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G666 - G674. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
