AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 280: G285-G290, 2001;
0193-1857/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (44)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barley, N. F.
Right arrow Articles by Walters, J. R. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barley, N. F.
Right arrow Articles by Walters, J. R. F.
Vol. 280, Issue 2, G285-G290, February 2001

Epithelial calcium transporter expression in human duodenum

Natalie F. Barley, Alison Howard, David O'Callaghan, Stephen Legon, and Julian R. F. Walters

Gastroenterology Section, Imperial College School of Medicine, Hammersmith Campus, London W12 0NN, United Kingdom

Calcium absorption in intestine and kidney involves transport through the apical membrane, cytoplasm, and basolateral membrane of the epithelial cells. Apical membrane calcium influx channels have recently been described in rabbit (epithelial calcium channel, ECaC) and rat (calcium transport protein, CaT1). We amplified from human duodenum a 446-base partial cDNA probe (ECAC2) having a predicted amino acid similarity of 97% to rat CaT1. Duodenum, but not ileum, colon, or kidney, expressed a 3-kb transcript. A larger transcript was also found in placenta and pancreas, and a different, faint transcript was found in brain. In duodenal biopsies from 20 normal volunteers, expression varied considerably but was not significantly correlated with vitamin D metabolites. This signal correlated with calbindin-D9k (r = 0.48, P < 0.05) and more strongly with the plasma membrane calcium ATPase PMCA1 (r = 0.83, P < 0.001). These data show that although individual variations in calcium channel transcripts are not vitamin D dependent, expression of genes governing apical entry and basolateral extrusion are tightly linked. This may account for some of the unexplained variability in calcium absorption.

intestine; absorption; 1,25-dihydroxycholecalciferol; brush-border membrane; calcium-adenosinetriphosphatase; calbindin-D9k; vitamin D


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G.-S. Lee and E.-B. Jeung
Uterine TRPV6 expression during the estrous cycle and pregnancy in a mouse model
Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E132 - E138.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H.-J. Kim, G.-S. Lee, Y.-K. Ji, K.-C. Choi, and E.-B. Jeung
Differential expression of uterine calcium transporter 1 and plasma membrane Ca2+ ATPase 1b during rat estrous cycle.
Am J Physiol Endocrinol Metab, August 1, 2006; 291(2): E234 - E241.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
K. Y. Renkema, T. Nijenhuis, B. C.J. van der Eerden, A. W.C.M. van der Kemp, H. Weinans, J. P.T.M. van Leeuwen, R. J.M. Bindels, and J. G.J. Hoenderop
Hypervitaminosis D Mediates Compensatory Ca2+ Hyperabsorption in TRPV5 Knockout Mice
J. Am. Soc. Nephrol., November 1, 2005; 16(11): 3188 - 3195.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. G. J. Hoenderop, B. Nilius, and R. J. M. Bindels
Calcium Absorption Across Epithelia
Physiol Rev, January 1, 2005; 85(1): 373 - 422.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. van Abel, J. G. J. Hoenderop, A. W. C. M. van der Kemp, J. P. T. M. van Leeuwen, and R. J. M. Bindels
Regulation of the epithelial Ca2+ channels in small intestine as studied by quantitative mRNA detection
Am J Physiol Gastrointest Liver Physiol, June 9, 2003; 285(1): G78 - G85.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
K. K. Frick and D. A. Bushinsky
Molecular Mechanisms of Primary Hypercalciuria
J. Am. Soc. Nephrol., April 1, 2003; 14(4): 1082 - 1095.
[Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. Moreau, A. Hamel, G. Daoud, L. Simoneau, and J. Lafond
Expression of Calcium Channels along the Differentiation of Cultured Trophoblast Cells from Human Term Placenta
Biol Reprod, November 1, 2002; 67(5): 1473 - 1479.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. C. Fleet, F. Eksir, K. W. Hance, and R. J. Wood
Vitamin D-inducible calcium transport and gene expression in three Caco-2 cell lines
Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G618 - G625.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
D. Muller, J. G. J. Hoenderop, C. H. van Os, and R. J. M. Bindels
The epithelial calcium channel, ECaC1: molecular details of a novel player in renal calcium handling
Nephrol. Dial. Transplant., July 1, 2001; 16(7): 1329 - 1335.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. J. Van Cromphaut, M. Dewerchin, J. G. J. Hoenderop, I. Stockmans, E. Van Herck, S. Kato, R. J. M. Bindels, D. Collen, P. Carmeliet, R. Bouillon, et al.
Duodenal calcium absorption in vitamin D receptor-knockout mice: Functional and molecular aspects
PNAS, November 6, 2001; 98(23): 13324 - 13329.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online