| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 3 Pediatrics, 2 Biochemistry, and 1 Nutrition, Université de Montréal, H3C 3J7; and 4 Department of Anatomy and Cellular Biology, Université de Sherbrooke, Montreal, Quebec, Canada H3T 1C5
To examine the multiple stages of lipoprotein packaging during development, we studied localization, ontogeny, and regulation of microsomal transfer protein (MTP), a crucial protein for lipid transport. With the use of immunofluorescence, MTP was identified in villus and crypt epithelial cells in different regions of human fetal intestine, including colon. Staining was detected as early as the 13th wk of gestation in all gut segments and was almost entirely confined to the columnar epithelial cells of the jejunum and colon. Unlike immunofluorescence, which provides qualitative but not quantitative information on MTP signal, enzymatic assays revealed a decreasing gradient from proximal small intestine to distal, as confirmed by immunoblot. Activity of MTP in small intestinal explants cultured for different incubation periods (0, 4, 8, and 24 h) peaked at 4 h but remained insensitive to different concentrations of oleic acid. Also, a trend toward increasing MTP activity was observed at 20-22 wk of gestation. Finally, in strong contrast to jejunal efficiency, colonic explants displayed impaired lipid production, apolipoprotein biogenesis, and lipoprotein assembly, in association with poor expression of MTP. These findings provide the first evidence that human fetal gut is able to express MTP and emphasize the distinct regional distribution, regulation by oleic acid, and ontogeny of MTP.
fat absorption; developmental biology; lipoprotein metabolism; intestinal transport
This article has been cited by other articles:
|
|
 |
|
  G. Mailhot, Z. Ravid, S. Barchi, A. Moreau, R. Rabasa-Lhoret, and E. Levy CFTR knockdown stimulates lipid synthesis and transport in intestinal Caco-2/15 cells Am J Physiol Gastrointest Liver Physiol, December 1, 2009; 297(6): G1239 - G1249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Black Development and Physiological Regulation of Intestinal Lipid Absorption. I. Development of intestinal lipid absorption: cellular events in chylomicron assembly and secretion Am J Physiol Gastrointest Liver Physiol, September 1, 2007; 293(3): G519 - G524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Peretti, C. C. Roy, E. Drouin, E. Seidman, P. Brochu, G. Casimir, and E. Levy Abnormal intracellular lipid processing contributes to fat malabsorption in cystic fibrosis patients Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G609 - G615. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Levy, D. Menard, I. Suc, E. Delvin, V. Marcil, L. Brissette, L. Thibault, and M. Bendayan Ontogeny, immunolocalisation, distribution and function of SR-BI in the human intestine J. Cell Sci., January 15, 2004; 117(2): 327 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lu, M. Huffman, Y. Yao, C. M. Mansbach II, X. Cheng, S. Meng, and D. D. Black Regulation of MTP expression in developing swine J. Lipid Res., August 1, 2002; 43(8): 1303 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Courtois, E. Delvin, M. Ledoux, E. Seidman, J.-C. Lavoie, and E. Levy The Antioxidant BHT Normalizes Some Oxidative Effects of Iron + Ascorbate on Lipid Metabolism in Caco-2 Cells J. Nutr., June 1, 2002; 132(6): 1289 - 1292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Levy, D. Menard, E. Delvin, S. Stan, G. Mitchell, M. Lambert, E. Ziv, J. C. Feoli-Fonseca, and E. Seidman The Polymorphism at Codon 54 of the FABP2 Gene Increases Fat Absorption in Human Intestinal Explants J. Biol. Chem., October 19, 2001; 276(43): 39679 - 39684. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
