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Am J Physiol Gastrointest Liver Physiol 280: G701-G709, 2001;
0193-1857/01 $5.00
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Vol. 280, Issue 4, G701-G709, April 2001

Expression and immunolocalization of aquaporin water channels in rat exocrine pancreas

Patricia T. Hurley1, Carole J. Ferguson1, Tae-Hwan Kwon2, Marie-Louise E. Andersen2, Alexander G. Norman1, Martin C. Steward1, Søren Nielsen2, and R. Maynard Case1

1 School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom; and 2 Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus, Denmark

Both the acinar and ductal cells of the pancreas secrete a near-isotonic fluid and may thus be sites of aquaporin (AQP) water channel expression. Northern blot analysis of mRNA from whole rat pancreas revealed high levels of AQP1 and AQP8 expression, whereas lower levels of AQP4 and AQP5 expression were just detectable by RT-PCR Southern blot analysis. Immunohistochemistry showed that AQP1 is localized in the microvasculature, whereas AQP8 is confined to the apical pole of the acinar cells. No labeling of acinar, ductal, or vascular tissue was detected with antibodies to AQP2-7. With immunoelectron microscopy, AQP8 labeling was observed not only at the apical membrane of the acinar cells but also among small intracellular vesicles in the subapical cytoplasm, suggesting that there may be regulated trafficking of AQP8 to the apical plasma membrane. To evaluate the contribution of AQPs to the membrane water permeability, video microscopy was used to measure the swelling of acinar cells in response to hypotonic stress. Osmotic water permeability was reduced by 90% following exposure to Hg2+. Since AQP8 is confined to the apical membrane, the marked effect of Hg2+ suggests that other water channels may be expressed in the basolateral membrane.

exocrine gland; fluid secretion; intracellular trafficking; water permeability


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