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Am J Physiol Gastrointest Liver Physiol 280: G1106-G1113, 2001;
0193-1857/01 $5.00
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Vol. 280, Issue 6, G1106-G1113, June 2001

Mechanism of action of baclofen in rat dorsal motor nucleus of the vagus

K. N. Browning and R. A. Travagli

Division of Gastroenterology and Department of Physiology, University of Michigan Medical Center, Ann Arbor, Michigan 48109

Using whole cell patch-clamp recordings, we investigated the effects of the GABAB receptor agonist baclofen in thin slices of rat brain stem containing identified gastric- or intestinal-projecting dorsal motor nucleus of the vagus (DMV) neurons. Perfusion with baclofen (0.1-100 µM) induced a concentration-dependent outward current (EC50, 3 µM) in 54% of DMV neurons with no apparent differences between gastric- and intestinal-projecting neurons. The outward current was attenuated by pretreatment with the selective GABAB antagonists saclofen and 2-hydroxysaclofen, but not by the synaptic blocker TTX, indicating a direct effect at GABAB receptors on DMV neurons. Using the selective ion channel blockers barium, nifedipine, and apamin, we showed that the outward current was due to effects on potassium and calcium currents as well as calcium-dependent potassium currents. The calcium-mediated components of the outward current were more prominent in intestinal-projecting neurons than in gastric-projecting neurons. These data indicate that although baclofen inhibits both intestinal- and gastric-projecting neurons in the rat DMV, its mechanism of action differs among the neuronal subpopulations.

brain stem; electrophysiology; gastrointestinal


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