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Am J Physiol Gastrointest Liver Physiol 280: G1137-G1144, 2001;
0193-1857/01 $5.00
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Vol. 280, Issue 6, G1137-G1144, June 2001

Regulation of apolipoprotein secretion by long-chain polyunsaturated fatty acids in newborn swine enterocytes

Heng Wang1, Song Lu2, Jianhui Du1, Ying Yao2, Helen M. Berschneider3, and Dennis D. Black2

2 Crippled Children's Foundation Research Center, Le Bonheur Children's Medical Center, University of Tennessee Health Science Center, Memphis, Tennessee 38103; 1 Arkansas Children's Hospital Research Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72202; and 3 College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606

Long-chain polyunsaturated fatty acids (LC-PUFA) are important in the development of the immature nervous system, and adding these fatty acids to infant formula has been proposed. To determine the effect of n-3 LC-PUFA on apolipoprotein secretion and lipid synthesis in newborn swine enterocytes, differentiated IPEC-1 cells were incubated for 24 h with docosahexaenoic acid (DHA; 22:6) or eicosapentaenoic acid (EPA; 20:5) complexed with albumin at a fatty acid concentration of 0.8 mM or albumin alone (control) added to the apical medium. Oleic acid (OA; 18:1) was used a control for lipid-labeling studies. Both DHA and EPA reduced apolipoprotein (apo) B secretion by one-half, whereas EPA increased apo A-I secretion. The increased apo A-I secretion occurred primarily in the high-density lipoprotein fraction. These changes in apoprotein secretion were not accompanied by significant changes in synthesis. Modest decreases in apo B mRNA levels were observed for DHA and EPA, whereas there were no changes in apo A-I mRNA abundance. EPA reduced cellular triacylglycerol labeling by one-half, and DHA and EPA decreased cellular phospholipid labeling compared with OA. Labeled triacylglycerol secretion was decreased 75% by EPA, and DHA doubled labeled phospholipid secretion. If present in vivo, these effects should be considered before supplementing infant formula with these fatty acids.

docosahexaenoic acid; eicosapentaenoic acid; messenger ribonucleic acid; phospholipid; triacylglycerol


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