AJP - GI Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 280: G1351-G1359, 2001;
0193-1857/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pérez-del-Pulgar, S.
Right arrow Articles by Rodés, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pérez-del-Pulgar, S.
Right arrow Articles by Rodés, J.
Vol. 280, Issue 6, G1351-G1359, June 2001

Neutrophil adhesion is impaired in the mesentery but not in the liver sinusoids of portal hypertensive rats

Sofía Pérez-del-Pulgar1, Pilar Pizcueta1, Pablo Engel2, Jaume Bosch1, and Joan Rodés1

1 Hepatic Hemodynamic Laboratory, Liver Unit, Institut Malalties Digestives, Hospital Clinic, and 2 Immunology Unit, Department of Cellular Biology and Pathology, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Medical School, University of Barcelona, 08036 Barcelona, Spain

Altered leukocyte/cytokine response to inflammation has been observed in human and experimental portal hypertension. The aim of this study was to characterize leukocyte adhesion in portal hypertensive (PPVL) rats stimulated with endotoxin. Leukocyte rolling, adhesion, and migration assessed by intravital microscopy were impaired in mesenteric venules after lipopolysaccharide administration (150 µg/kg) in PPVL vs. sham-operated rats. Analysis of leukocyte L-selectin expression and soluble L-selectin showed that this defective adhesion was related to increased L-selectin shedding. In vitro experiments using isolated leukocytes treated with phorbol 12-myristate 13-acetate showed that monocytes and neutrophils but not lymphocytes were hyperreactive to cell activation, as measured by CD11b overexpression and increased L-selectin shedding in PPVL rats. However, neutrophil emigration in liver sinusoids and in the lung 3 h after endotoxin injection were similar in both groups of animals. Thus the alterations in leukocyte activation and adhesion molecule expression observed in this study may contribute to a better understanding of the higher susceptibility and severity of bacterial infections in cirrhotic patients with portal hypertension.

portal hypertension; sepsis; L-selectin; cirrhosis; adhesion molecules


This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
S. Mortier, A. S. de Vriese, R. M. McLoughlin, N. Topley, T. P. Schaub, J. Passlick-Deetjen, and N. H. Lameire
Effects of Conventional and New Peritoneal Dialysis Fluids on Leukocyte Recruitment in the Rat Peritoneal Membrane
J. Am. Soc. Nephrol., May 1, 2003; 14(5): 1296 - 1306.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online