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Am J Physiol Gastrointest Liver Physiol 281: G333-G341, 2001;
0193-1857/01 $5.00
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Vol. 281, Issue 2, G333-G341, August 2001

Nonselective cation channel as a Ca2+ influx pathway in pepsinogen-secreting cells of bullfrog esophagus

Seiichiro Kimura1, Hiroshi Mieno2, Kenji Tamaki3, Masaki Inoue4, and Kazuaki Chayama5

1 Saiseikai Kure Hospital, Kure City, Hiroshima 737-0821; 2 Hiroshima General Hospital of the West Railroad Company, Hiroshima City, Hiroshima 732-0057; 3 Chugoku Rosai Hospital, Kure City, Hiroshima 737-0193; and 4 Department of Geriatric Medicine and First 5 Department of Internal Medicine, Institute of Health Sciences, Hiroshima University School of Medicine, Hiroshima 734-8551, Japan

In pepsinogen-secreting cells of bullfrog (Rana catesbeiana), recent evidence suggests that Ca2+ release from internal stores followed by Ca2+ influx across the plasma membrane elicits pepsinogen secretion. Such a Ca2+ influx could be carried by a background current, potentiated by bombesin, that was found in these cells using the whole cell patch-clamp technique. The permeability ratio of Cs+-Rb+-K+-Na+-Li+-N-methyl-D-glucamine+-Ca2+ was 1.01:1:1:0.86:0.72:0.54:0.34. The current was almost totally blocked by the nonselective cation channel blockers La3+ (0.1 mM) and Gd3+ (0.1 mM) and was activated by intracellular Ca2+. These properties demonstrated that the current, which was activated by bombesin, was a nonselective cation current. At the same time, Gd3+ suppressed pepsinogen secretion by 29 ± 5.6% in isolated pepsinogen-secreting glands. These results are in accord with the idea that a nonselective cation channel in pepsinogen-secreting cells plays a role as a Ca2+ influx pathway leading to secretion of pepsinogen in bullfrog esophageal mucosa.

patch clamp; bombesin; nifedipine; lanthanum; gadolinium; calcium ion


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