AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 281: G365-G370, 2001;
0193-1857/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ugawa, S.
Right arrow Articles by Shimada, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ugawa, S.
Right arrow Articles by Shimada, S.
Vol. 281, Issue 2, G365-G370, August 2001

Characterization of a mouse colonic system B0+ amino acid transporter related to amino acid absorption in colon

Shinya Ugawa, Yoko Sunouchi, Takashi Ueda, Eri Takahashi, Yoshitsugu Saishin, and Shoichi Shimada

Department of Anatomy II, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan

Previous experiments have shown that an amino acid transport system B0+ transporter in cultured colonic epithelial cells mediates amino acid absorption. Here we describe the cloning and functional characterization of a system B0+ transporter selectively expressed in the colon. Using the combination of an expressed sequence tag database search and RT-PCR approaches, we cloned a mouse colonic amino acid transporter, designated mCATB0+. Northern blot analysis revealed that mCATB0+ was selectively expressed in the large intestine. In situ hybridization showed the mCATB0+ mRNA to be localized in absorptive epithelial cells. When expressed in Xenopus oocytes, mCATB0+ exhibited a Na+-dependent stereoselective uptake and a broad specificity for neutral and cationic amino acids, which is characteristic of amino acid transport system B0+. In vivo [3H]glycine uptake assay demonstrated that a system B0+-like transporter protein was expressed on the apical surface of the colonic absorptive cells. Our data suggest that a mouse colonic amino acid transporter mCATB0+ may absorb amino acids from the intestinal contents in the colon.

cDNA cloning; absorptive epithelial cell


This article has been cited by other articles:


Home page
 JPEN J Parenter Enteral NutrHome page
M. W. Schaart, A. C. J. M. de Bruijn, D. Tibboel, I. B. Renes, and J. B. van Goudoever
Dietary Protein Absorption of the Small Intestine in Human Neonates
JPEN J Parenter Enteral Nutr, November 1, 2007; 31(6): 482 - 486.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
P. J Moughan, C. A Butts, A. M Rowan, and A. Deglaire
Dietary peptides increase endogenous amino acid losses from the gut in adults
Am. J. Clinical Nutrition, June 1, 2005; 81(6): 1359 - 1365.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. L. Sloan, B. R. Grubb, and S. Mager
Expression of the amino acid transporter ATB0+ in lung: possible role in luminal protein removal
Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L39 - L49.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. A. Cuff, D. W. Lambert, and S. P. Shirazi-Beechey
Substrate-induced regulation of the human colonic monocarboxylate transporter, MCT1
J. Physiol., March 1, 2002; 539(2): 361 - 371.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online