AJP - GI AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 281: G697-G704, 2001;
0193-1857/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (30)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Groneberg, D. A.
Right arrow Articles by Daniel, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Groneberg, D. A.
Right arrow Articles by Daniel, H.
Vol. 281, Issue 3, G697-G704, September 2001

Intestinal peptide transport: ex vivo uptake studies and localization of peptide carrier PEPT1

David A. Groneberg1,2, Frank Döring3, Paul R. Eynott4, Axel Fischer1, and Hannelore Daniel3

1 Department of Pediatrics, Charité Campus Virchow, Humboldt University, 13353 Berlin; 2 Institute for Anatomy and Cell Biology, Faculty of Medicine, Justus Liebig University, 35385 Giessen; 3 Institute of Nutritional Sciences, Technical University of Munich, 85350 Freising-Weihenstephan, Germany; and 4 National Heart and Lung Institute, Imperial College of Science, Technology, and Medicine, London SW3 6LY, United Kingdom

The nature of protein breakdown products and peptidomimetic drugs such as beta -lactams is crucial for their transmembrane transport across apical enterocyte membranes, which is accomplished by the pH-dependent high-capacity oligopeptide transporter PEPT1. To visualize oligopeptide transporter-mediated uptake of oligopeptides, an ex vivo assay using the fluorophore-conjugated dipeptide derivative D-Ala-Lys-Nepsilon -7-amino-4-methylcoumarin-3-acetic acid (D-Ala-Lys-AMCA) was established in the murine small intestine and compared with immunohistochemistry for PEPT1 in murine and human small intestine. D-Ala-Lys-AMCA was accumulated by enterocytes throughout all segments of the murine small intestine, with decreasing intensity from the top to the base of the villi. Goblet cells did not show specific uptake. Inhibition studies revealed competitive inhibition by the beta -lactam cefadroxil, the angiotensin-converting enzyme inhibitor captopril, and the dipeptide glycyl-glutamine. Controls were performed using either the inhibitor diethylpyrocarbonate or an incubation temperature of 4°C to exclude unspecific uptake. Immunohistochemistry for PEPT1 localized immunoreactivity to the enterocytes, with the highest intensity at the apical membrane. This is the first study that visualizes dipeptide transport across the mammalian intestine and indicates that uptake assays using D-Ala-Lys-AMCA might be useful for characterizing PEPT1-specific substrates or inhibitors.

immunohistochemistry; oligopeptide; human; mouse


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Choi, M. Lee, A. L. Shiu, S. J. Yo, and G. W. Aponte
Identification of a protein hydrolysate responsive G protein-coupled receptor in enterocytes
Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G98 - G112.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Y. Nasser, E. Fernandez, C. M. Keenan, W. Ho, L. D. Oland, L. A. Tibbles, M. Schemann, W. K. MacNaughton, A. Ruhl, and K. A. Sharkey
Role of enteric glia in intestinal physiology: effects of the gliotoxin fluorocitrate on motor and secretory function
Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G912 - G927.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. H. Dave, N. Schulz, M. Zecevic, C. A. Wagner, and F. Verrey
Expression of heteromeric amino acid transporters along the murine intestine
J. Physiol., July 15, 2004; 558(2): 597 - 610.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. A. Groneberg, A. Fischer, K. F. Chung, and H. Daniel
Molecular Mechanisms of Pulmonary Peptidomimetic Drug and Peptide Transport
Am. J. Respir. Cell Mol. Biol., March 1, 2004; 30(3): 251 - 260.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Daniel and I. Rubio-Aliaga
An update on renal peptide transporters
Am J Physiol Renal Physiol, May 1, 2003; 284(5): F885 - F892.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
I. Knutter, I. Rubio-Aliaga, M. Boll, G. Hause, H. Daniel, K. Neubert, and M. Brandsch
H+-peptide cotransport in the human bile duct epithelium cell line SK-ChA-1
Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G222 - G229.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-C. Gevrey, M. Cordier-Bussat, E. Nemoz-Gaillard, J.-A. Chayvialle, and J. Abello
Co-requirement of Cyclic AMP- and Calcium-dependent Protein Kinases for Transcriptional Activation of Cholecystokinin Gene by Protein Hydrolysates
J. Biol. Chem., June 14, 2002; 277(25): 22407 - 22413.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
D. A. Groneberg, C. Grosse-Siestrup, and A. Fischer
In Vitro Models to Study Hepatotoxicity
Toxicol Pathol, April 1, 2002; 30(3): 394 - 399.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online