AJP - GI Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 281: G890-G898, 2001;
0193-1857/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Savkovic, S. D.
Right arrow Articles by Hecht, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Savkovic, S. D.
Right arrow Articles by Hecht, G.
Vol. 281, Issue 4, G890-G898, October 2001

EPEC-activated ERK1/2 participate in inflammatory response but not tight junction barrier disruption

Suzana D. Savkovic, Akila Ramaswamy, Athanasia Koutsouris, and Gail Hecht

Department of Medicine, Section of Digestive and Liver Diseases, University of Illinois, West Side Veterans Affairs Medical Center, Chicago, Illinois 60612

Enteropathogenic Escherichia coli (EPEC) alters many functions of the host intestinal epithelia. Inflammation is initiated by activation of nuclear factor (NF)-kappa B, and paracellular permeability is enhanced via a Ca2+- and myosin light-chain kinase (MLCK)-dependent pathway. The aims of this study were to identify signaling pathways by which EPEC triggers inflammation and to determine whether these pathways parallel or diverge from those that alter permeability. EPEC-induced phosphorylation and degradation of the primary inhibitor of NF-kappa B (Ikappa Balpha ) were tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta independent. In contrast to Salmonella typhimurium, EPEC-stimulated Ikappa Balpha degradation and IL-8 expression did not require Ca2+. Instead, extracellular signal-regulated kinase (ERK)-1/2 was significantly and rapidly activated. ERK1/2 inhibitors attenuated Ikappa Balpha degradation and IL-8 expression. Although ERK1/2 can activate MLCK, its inhibition had no impact on EPEC disruption of the tight junction barrier. In conclusion, EPEC-induced inflammation 1) is TNF-alpha and IL-1beta receptor independent, 2) utilizes pathways differently from S. typhimurium, 3) requires ERK1/2, and 4) employs signals that are distinct from those that alter permeability. This is the first time that EPEC-activated signaling cascades have been linked to independent functional consequences.

permeability; nuclear factor-kappa B; primary inhibitor of nuclear factor-kappa B; interleukin-8; enteric pathogens; enteropathogenic Escherichia coli; extracellular signal-regulated kinase-1/2


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Oshima, H. Miwa, and T. Joh
Aspirin induces gastric epithelial barrier dysfunction by activating p38 MAPK via claudin-7
Am J Physiol Cell Physiol, September 1, 2008; 295(3): C800 - C806.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. V. Zurawski, K. L. Mumy, L. Badea, J. A. Prentice, E. L. Hartland, B. A. McCormick, and A. T. Maurelli
The NleE/OspZ Family of Effector Proteins Is Required for Polymorphonuclear Transepithelial Migration, a Characteristic Shared by Enteropathogenic Escherichia coli and Shigella flexneri Infections
Infect. Immun., January 1, 2008; 76(1): 369 - 379.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. L. Roxas, A. Koutsouris, and V. K. Viswanathan
Enteropathogenic Escherichia coli-Induced Epidermal Growth Factor Receptor Activation Contributes to Physiological Alterations in Intestinal Epithelial Cells
Infect. Immun., May 1, 2007; 75(5): 2316 - 2324.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. T. Chiu, W. Y. Leung, M. P. Moyer, R. M. Strieter, and E. Rozengurt
Protein kinase D2 mediates lysophosphatidic acid-induced interleukin 8 production in nontransformed human colonic epithelial cells through NF-{kappa}B
Am J Physiol Cell Physiol, February 1, 2007; 292(2): C767 - C777.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. L. Chen, Z. Ge, J. G. Fox, and D. B. Schauer
Disruption of Tight Junctions and Induction of Proinflammatory Cytokine Responses in Colonic Epithelial Cells by Campylobacter jejuni
Infect. Immun., December 1, 2006; 74(12): 6581 - 6589.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. A. Guttman, F. N. Samji, Y. Li, A. W. Vogl, and B. B. Finlay
Evidence that Tight Junctions Are Disrupted Due to Intimate Bacterial Contact and Not Inflammation during Attaching and Effacing Pathogen Infection In Vivo
Infect. Immun., November 1, 2006; 74(11): 6075 - 6084.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. A. Johanesen and M. B. Dwinell
Flagellin-Independent Regulation of Chemokine Host Defense in Campylobacter jejuni-Infected Intestinal Epithelium.
Infect. Immun., June 1, 2006; 74(6): 3437 - 3447.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. Sharma, S. Tesfay, F. L. Tomson, R. P. Kanteti, V. K. Viswanathan, and G. Hecht
Balance of bacterial pro- and anti-inflammatory mediators dictates net effect of enteropathogenic Escherichia coli on intestinal epithelial cells
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G685 - G694.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
G. Hecht, K. Hodges, R. K. Gill, F. Kear, S. Tyagi, J. Malakooti, K. Ramaswamy, and P. K. Dudeja
Differential regulation of Na+/H+ exchange isoform activities by enteropathogenic E. coli in human intestinal epithelial cells
Am J Physiol Gastrointest Liver Physiol, August 1, 2004; 287(2): G370 - G378.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. D. Savkovic, A. Koutsouris, and G. Hecht
PKC{zeta} participates in activation of inflammatory response induced by enteropathogenic E. coli
Am J Physiol Cell Physiol, September 1, 2003; 285(3): C512 - C521.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
F. Betis, P. Brest, V. Hofman, J. Guignot, I. Kansau, B. Rossi, A. Servin, and P. Hofman
Afa/Dr Diffusely Adhering Escherichia coli Infection in T84 Cell Monolayers Induces Increased Neutrophil Transepithelial Migration, Which in Turn Promotes Cytokine-Dependent Upregulation of Decay-Accelerating Factor (CD55), the Receptor for Afa/Dr Adhesins
Infect. Immun., April 1, 2003; 71(4): 1774 - 1783.
[Abstract] [Full Text]

Home page
 GutHome page
J Berkes, V K Viswanathan, S D Savkovic, and G Hecht
Intestinal epithelial responses to enteric pathogens: effects on the tight junction barrier, ion transport, and inflammation
Gut, March 1, 2003; 52(3): 439 - 451.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. J. Keely and K. E. Barrett
p38 mitogen-activated protein kinase inhibits calcium-dependent chloride secretion in T84 colonic epithelial cells
Am J Physiol Cell Physiol, February 1, 2003; 284(2): C339 - C348.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. A. Vallance, W. Deng, M. De Grado, C. Chan, K. Jacobson, and B. B. Finlay
Modulation of Inducible Nitric Oxide Synthase Expression by the Attaching and Effacing Bacterial Pathogen Citrobacter rodentium in Infected Mice
Infect. Immun., November 1, 2002; 70(11): 6424 - 6435.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online