| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Joint Clinical Sciences Program, The Queensland Institute of Medical Research and The University of Queensland, PO Royal Brisbane Hospital and 5 Department of Pediatrics and Child Health, University of Queensland, Brisbane, Queensland 4029; and 4 Department of Medicine, The University of Western Australia, Fremantle Hospital, Fremantle, Western Australia 6160, Australia; 2 Department of Nutritional Sciences, University of California, Berkeley, California 94720; and 3 Department of Molecular Medicine, King's College, London SE59NU, United Kingdom
The membrane-bound ceruloplasmin homolog hephaestin plays a critical role in intestinal iron absorption. The aims of this study were to clone the rat hephaestin gene and to examine its expression in the gastrointestinal tract in relation to other genes encoding iron transport proteins. The rat hephaestin gene was isolated from intestinal mRNA and was found to encode a protein 96% identical to mouse hephaestin. Analysis by ribonuclease protection assay and Western blotting showed that hephaestin was expressed at high levels throughout the small intestine and colon. Immunofluorescence localized the hephaestin protein to the mature villus enterocytes with little or no expression in the crypts. Variations in iron status had a small but nonsignificant effect on hephaestin expression in the duodenum. The high sequence conservation between rat and mouse hephaestin is consistent with this protein playing a central role in intestinal iron absorption, although its precise function remains to be determined.
divalent metal transporter-1; Ireg1; absorption; intestine
This article has been cited by other articles:
|
|
 |
|
  C. D. San Martin, C. Garri, F. Pizarro, T. Walter, E. C. Theil, and M. T. Nunez Caco-2 Intestinal Epithelial Cells Absorb Soybean Ferritin by {micro}2 (AP2)-Dependent Endocytosis J. Nutr., April 1, 2008; 138(4): 659 - 666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Boult, K. Roberts, M. J. Brookes, S. Hughes, J. P. Bury, S. S. Cross, G. J. Anderson, R. Spychal, T. Iqbal, and C. Tselepis Overexpression of Cellular Iron Import Proteins Is Associated with Malignant Progression of Esophageal Adenocarcinoma Clin. Cancer Res., January 15, 2008; 14(2): 379 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Frazer, S. J. Wilkins, and G. J. Anderson Elevated iron absorption in the neonatal rat reflects high expression of iron transport genes in the distal alimentary tract Am J Physiol Gastrointest Liver Physiol, September 1, 2007; 293(3): G525 - G531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Markel, P. R. Crisostomo, M. Wang, C. M. Herring, K. K. Meldrum, K. D. Lillemoe, and D. R. Meldrum The struggle for iron: gastrointestinal microbes modulate the host immune response during infection J. Leukoc. Biol., February 1, 2007; 81(2): 393 - 400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M J Brookes, S Hughes, F E Turner, G Reynolds, N Sharma, T Ismail, G Berx, A T McKie, N Hotchin, G J Anderson, et al. Modulation of iron transport proteins in human colorectal carcinogenesis Gut, October 1, 2006; 55(10): 1449 - 1460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Chicault, B. Toutain, A. Monnier, M. Aubry, P. Fergelot, A. L. Treut, M.-D. Galibert, and J. Mosser Iron-related transcriptomic variations in CaCo-2 cells, an in vitro model of intestinal absorptive cells Physiol Genomics, September 14, 2006; 26(1): 55 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Fleming and R. S. Britton Iron Imports. VI. HFE and regulation of intestinal iron absorption Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G590 - G594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K N Millard, D M Frazer, S J Wilkins, and G J Anderson Changes in the expression of intestinal iron transport and hepatic regulatory molecules explain the enhanced iron absorption associated with pregnancy in the rat Gut, May 1, 2004; 53(5): 655 - 660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-y. Yeh, M. Yeh, and J. Glass Hepcidin regulation of ferroportin 1 expression in the liver and intestine of the rat Am J Physiol Gastrointest Liver Physiol, March 1, 2004; 286(3): G385 - G394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y M Kuo, T Su, H Chen, Z Attieh, B A Syed, A T McKie, G J Anderson, J Gitschier, and C D Vulpe Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral intestinal iron transport, in the sex linked anaemia mouse Gut, February 1, 2004; 53(2): 201 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Thomas and P. S. Oates Copper deficiency increases iron absorption in the rat Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G789 - G795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Chen, T. Su, Z. K. Attieh, T. C. Fox, A. T. McKie, G. J. Anderson, and C. D. Vulpe Systemic regulation of Hephaestin and Ireg1 revealed in studies of genetic and nutritional iron deficiency Blood, September 1, 2003; 102(5): 1893 - 1899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Fleet, L. Wang, O. Vitek, B. A. Craig, and H. J. Edenberg Gene expression profiling of Caco-2 BBe cells suggests a role for specific signaling pathways during intestinal differentiation Physiol Genomics, March 18, 2003; 13(1): 57 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D M Frazer, S J Wilkins, E M Becker, T L Murphy, C D Vulpe, A T McKie, and G J Anderson A rapid decrease in the expression of DMT1 and Dcytb but not Ireg1 or hephaestin explains the mucosal block phenomenon of iron absorption Gut, March 1, 2003; 52(3): 340 - 346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. N. Patel, R. J. Dunn, S. Y. Jeong, Q. Zhu, J.-P. Julien, and S. David Ceruloplasmin Regulates Iron Levels in the CNS and Prevents Free Radical Injury J. Neurosci., August 1, 2002; 22(15): 6578 - 6586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Thomas and P. S. Oates IEC-6 Cells Are an Appropriate Model of Intestinal Iron Absorption in Rats J. Nutr., April 1, 2002; 132(4): 680 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Syed, N. J. Beaumont, A. Patel, C. E. Naylor, H. K. Bayele, C. L. Joannou, P. S.N. Rowe, R. W. Evans, and S. K. S. Srai Analysis of the human hephaestin gene and protein: comparative modelling of the N-terminus ecto-domain based upon ceruloplasmin Protein Eng. Des. Sel., March 1, 2002; 15(3): 205 - 214. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
