AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 281: G1140-G1150, 2001;
0193-1857/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (33)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Edde, L.
Right arrow Articles by Sherman, M. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Edde, L.
Right arrow Articles by Sherman, M. P.
Vol. 281, Issue 5, G1140-G1150, November 2001

TRANSLATIONAL PHYSIOLOGY
Lactoferrin protects neonatal rats from gut-related systemic infection

Lynn Edde1,5,*, Ronaldo B. Hipolito2,*, Freda F. Y. Hwang2, Denis R. Headon3, Robert A. Shalwitz4, and Michael P. Sherman2,5

1 Department of Pediatrics, University of Arizona, Tucson, Arizona 85724; 2 Department of Pediatrics, University of California, Davis, California 95616; 3 Agennix Incorporated, Houston, Texas 77046; 4 Ross Products Division, Abbott Laboratories, Columbus, Ohio 43215; and 5 Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030

Lactoferrin is a milk protein that reportedly protects infants from gut-related, systemic infection. Proof for this concept is limited and was addressed during in vivo and in vitro studies. Neonatal rats pretreated orally with recombinant human lactoferrin (rh-LF) had less bacteremia and lower disease severity scores (P < 0.001) after intestinal infection with Escherichia coli. Control animals had 1,000-fold more colony-forming units of E. coli per milliliter of blood than treated animals (P < 0.001). Liver cultures from control animals had a twofold increase in bacterial counts compared with cultures from rh-LF-treated pups (P < 0.02). Oral therapy with rh-LF + FeSO4 did not alter the protective effect. In vitro studies confirmed that rh-LF interacted with the infecting bacterium and rat macrophages. An in vitro assay showed that rh-LF did not kill E. coli, but a combination of rh-LF + lysozyme was microbicidal. In vitro studies showed that rat macrophages released escalating amounts of nitric oxide and tumor necrosis factor-alpha when stimulated with increasing concentrations of rh-LF. The in vitro studies suggest that rh-LF may act with other "natural peptide antibiotics" or may prime macrophages to kill E. coli in vivo.

bacteremia; Escherichia coli; disease severity score; human lysozyme; macrophage-related priming or activation; recombinant human lactoferrin

* L. Edde and R. B. Hipolito contributed equally to this investigation.




This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
M. P. Venkatesh and L. Rong
Human recombinant lactoferrin acts synergistically with antimicrobials commonly used in neonatal practice against coagulase-negative staphylococci and Candida albicans causing neonatal sepsis
J. Med. Microbiol., September 1, 2008; 57(9): 1113 - 1121.
[Abstract] [Full Text] [PDF]

Home page
 J Trop PediatrHome page
K. Israel-Ballard, A. Coutsoudis, C. J. Chantry, A. W. Sturm, F. Karim, L. Sibeko, and B. Abrams
Bacterial Safety of Flash-heated and Unheated Expressed Breastmilk during Storage
J Trop Pediatr, December 1, 2006; 52(6): 399 - 405.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. J. Ochoa, M. Noguera-Obenza, F. Ebel, C. A. Guzman, H. F. Gomez, and T. G. Cleary
Lactoferrin Impairs Type III Secretory System Function in Enteropathogenic Escherichia coli
Infect. Immun., September 1, 2003; 71(9): 5149 - 5155.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
B. Lonnerdal
Nutritional and physiologic significance of human milk proteins
Am. J. Clinical Nutrition, June 1, 2003; 77 (6): 1537S - 1543S.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. E. Hall
The Promise of Translational Physiology
J Neurophysiol, February 1, 2003; 89(2): 646 - 647.
[Full Text] [PDF]

Home page
 NeoReviewsHome page
Y. Huang, K. Liboni, and J. Neu
Immunonutrients and the Critically Ill Neonate
NeoReviews, January 1, 2003; 4(1): e20 - 25.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. E. Hall
The promise of translational physiology
Am J Physiol Endocrinol Metab, August 1, 2002; 283(2): E193 - E194.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. E. Hall
The promise of translational physiology
Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L235 - L236.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. E. Hall
The promise of translational physiology
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2002; 282(5): R1259 - R1260.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. E. Hall
The promise of translational physiology
Am J Physiol Heart Circ Physiol, March 1, 2002; 282(3): H803 - H804.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. E. Hall
The promise of translational physiology
J Appl Physiol, February 1, 2002; 92(2): 425 - 426.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. E. Hall
The promise of translational physiology
Am J Physiol Gastrointest Liver Physiol, November 1, 2001; 281(5): G1127 - G1128.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. E. Hall
The promise of translational physiology
Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1411 - C1412.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online