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Am J Physiol Gastrointest Liver Physiol 281: G1264-G1270, 2001;
0193-1857/01 $5.00
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Vol. 281, Issue 5, G1264-G1270, November 2001

Myogenic NOS and endogenous NO production are defective in colon from dystrophic (mdx) mice

Flavia Mulè1, Maria Giuliana Vannucchi2, Letizia Corsani2, Rosa Serio3, and Maria Simonetta Faussone-Pellegrini2

1 Dipartimento Farmaco-Biologico, Università della Calabria, 87036 Arcavacata di Rende (CS); 2 Dipartimento di Anatomia, Istologia, e Medicina Legale, Università di Firenze, 50134 Florence; and 3 Dipartimento di Biologia cellulare e dello Sviluppo, Università di Palermo, 90128 Palermo, Italy

The aim of the present study was to evaluate whether alterations in the distribution and/or function of nitric oxide synthase (NOS) could be involved in the development of the spontaneous mechanical tone observed in colon from dystrophic (mdx) mice. By recording the intraluminal pressure of isolated colon from normal mice, we showed that Nomega -nitro- L-arginine methyl ester (L-NAME) increased the tone, even in the presence of tetrodotoxin. The effect was prevented by L-arginine, nifedipine, or Ca2+-free solution. In colon from mdx mice, L-NAME was ineffective. Immunohistochemistry revealed that the presence and distribution of neuronal (nNOS), endothelial, and inducible NOS isoforms in smooth muscle cells and neurons of colon from mdx mice were the same as in controls. However, the expression of myogenic nNOS was markedly reduced in mdx mice. We conclude that there is a myogenic NOS in mouse colon that can tonically produce nitric oxide to limit influx of Ca2+ through L-type voltage-dependent channels and modulate the mechanical tone. This mechanism appears to be defective in mdx mice.

nitric oxide synthase; nitric oxide; Duchenne muscular dystrophy; intestinal smooth muscle; spontaneous tone; immunohistochemistry


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M. C. Baccari, S. Nistri, M. G. Vannucchi, F. Calamai, and D. Bani
Reversal by relaxin of altered ileal spontaneous contractions in dystrophic (mdx) mice through a nitric oxide-mediated mechanism
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R662 - R668.
[Abstract] [Full Text] [PDF]


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