| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Molecular and Cellular Physiology and 2 Department of Medicine, Center of Excellence in Arthritis and Rheumatology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130-3932
Previous studies have revealed that the expression of several endothelial cell adhesion molecules [e.g., intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and mucosal addressin cell adhesion molecule 1 (MAdCAM-1)] is dramatically elevated in the chronically inflamed colonic vasculature of severe combined immunodeficient (SCID) mice reconstituted with congenic CD4+, CD45RBhigh T lymphocytes. The objective of this study was to define the contribution of different endothelial cell adhesion molecules to the lymphocyte-endothelial cell (L/E) adhesion observed in the colonic microvasculature in this experimental model of inflammatory bowel disease. Fluorescently labeled T lymphocytes, isolated from spleens of normal BALB/C mice, were injected intravenously into SCID mice that had been reconstituted with CD4+, CD45RBhigh T lymphocytes either before (3 wk after reconstitution) or after (7 wk postreconstitution) the onset of clinical signs of colitis (i.e., diarrhea, loss of body wt). Intravital fluorescence microscopy was used to quantify L/E adhesion in different-sized venules of the colonic submucosa during the development of colitis. L/E adhesion was noted in some segments of the vasculature in precolitic SCID mice (3 wk after reconstitution) but not in similar-sized vessels of control (wild type and SCID) mice. L/E adhesion was observed in a greater proportion of venules and occurred with greater intensity in the mucosa of colitic mice (7 wk postreconstitution). Pretreatment with a blocking monoclonal antibody against MAdCAM-1, but not ICAM-1 or VCAM-1, significantly and profoundly reduced L/E adhesion in colitic mice. Immunohistochemical staining also revealed the localization of T cells on colonic endothelial cells expressing MAdCAM-1. These findings indicate that MAdCAM-1 is largely responsible for recruiting T lymphocytes into inflamed colonic tissue.
intercellular adhesion molecule 1; vascular cell adhesion molecule 1; inflammatory bowel disease; microcirculation; mucosal addressin cell adhesion molecule
This article has been cited by other articles:
|
|
 |
|
  D. V. Ostanin, K. L. Furr, K. P. Pavlick, L. Gray, C. G. Kevil, D. Shukla, D. D'Souza, J. M. Hoffman, and M. B. Grisham T cell-associated CD18 but not CD62L, ICAM-1, or PSGL-1 is required for the induction of chronic colitis Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1706 - G1714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, E. P. Wojcikiewicz, and V. T. Moy Dynamic Adhesion of T Lymphocytes to Endothelial Cells Revealed by Atomic Force Microscopy Experimental Biology and Medicine, September 1, 2006; 231(8): 1306 - 1312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Anthoni, M. G. Laukoetter, E. Rijcken, T. Vowinkel, R. Mennigen, S. Muller, N. Senninger, J. Russell, J. Jauch, J. Bergmann, et al. Mechanisms underlying the anti-inflammatory actions of boswellic acid derivatives in experimental colitis Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1131 - G1137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rivera-Nieves, T. Olson, G. Bamias, A. Bruce, M. Solga, R. F. Knight, S. Hoang, F. Cominelli, and K. Ley L-Selectin, {alpha}4{beta}1, and {alpha}4{beta}7 Integrins Participate in CD4+ T Cell Recruitment to Chronically Inflamed Small Intestine J. Immunol., February 15, 2005; 174(4): 2343 - 2352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sasaki, J. W. Elrod, P. Jordan, M. Itoh, T. Joh, A. Minagar, and J. S. Alexander CYP450 dietary inhibitors attenuate TNF-{alpha}-stimulated endothelial molecule expression and leukocyte adhesion Am J Physiol Cell Physiol, April 1, 2004; 286(4): C931 - C939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sasaki, S. Bharwani, P. Jordan, T. Joh, K. Manas, A. Warren, H. Harada, P. Carter, J. W. Elrod, M. Wolcott, et al. The 3-Hydroxy-3-methylglutaryl-CoA Reductase Inhibitor Pravastatin Reduces Disease Activity and Inflammation in Dextran-Sulfate Induced Colitis J. Pharmacol. Exp. Ther., April 1, 2003; 305(1): 78 - 85. [Abstract] [Full Text]
|
|
|
|
|
|
C. Watanabe, S. Miura, R. Hokari, K. Teramoto, T. Ogino, S. Komoto, Y. Hara, S. Koseki, Y. Tsuzuki, H. Nagata, et al. Spatial heterogeneity of TNF-alpha -induced T cell migration to colonic mucosa is mediated by MAdCAM-1 and VCAM-1 Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1379 - G1387. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
