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Am J Physiol Gastrointest Liver Physiol 281: G1316-G1321, 2001;
0193-1857/01 $5.00
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Vol. 281, Issue 5, G1316-G1321, November 2001

Mucosal acid challenge activates nitrergic neurons in myenteric plexus of rat stomach

Rudolf Schicho1, Michael Schemann2, Peter Holzer1, and Irmgard T. Lippe1

1 Department of Experimental and Clinical Pharmacology, University of Graz, A-8010 Graz, Austria; and 2 Department of Physiology, School of Veterinary Medicine, D-30173 Hannover, Germany

We tested the hypothesis that intrinsic neurons of the rat gastric myenteric plexus can be activated by an acid (HCl) challenge of the mucosa. Activated neurons were visualized by immunohistochemical detection of c-Fos, a marker for neuronal excitation. The neurochemical identity of the neurons activated by the HCl challenge was determined by colocalizing c-Fos with a marker for excitatory pathways, choline acetyltransferase (ChAT), and a marker for inhibitory pathways, nitric oxide synthase (NOS). Two hours after intragastric administration of HCl or saline, stomachs were removed and immunofluorescence triple labeling of myenteric neurons was carried out on whole mount preparations. Treatment with 0.35, 0.5, and 0.7 M HCl induced c-Fos in 8%, 56%, and 64%, respectively, of NOS-positive but not ChAT-positive neurons. c-Fos was also seen in glial cells of HCl-treated rats, whereas in saline-treated animals c-Fos was absent from the myenteric plexus. HCl treatment did not change the proportion of ChAT- and NOS-immunoreactive neurons in the myenteric ganglia. It is concluded that gastric acid challenge concentration-dependently stimulates a subpopulation of nitrergic, but not cholinergic, myenteric plexus neurons, which may play a role in muscle relaxation, vasodilatation, and/or secretion.

enteric nervous system; gastric mucosal acid challenge; c-Fos; triple labeling


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