AJP - GI Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 281: G1494-G1501, 2001;
0193-1857/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smid, S. D.
Right arrow Articles by Blackshaw, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smid, S. D.
Right arrow Articles by Blackshaw, L. A.
Vol. 281, Issue 6, G1494-G1501, December 2001

GABABR expressed on vagal afferent neurones inhibit gastric mechanosensitivity in ferret proximal stomach

Scott D. Smid, Richard L. Young, Nicole J. Cooper, and L. Ashley Blackshaw

Nerve-Gut Research Laboratory, Level 1 Hanson Centre, Adelaide SA 5000, Australia

GABAB-receptor (GABABR) agonists reduce transient lower esophageal sphincter relaxation (TLESR) and reflux episodes through an action on vagal pathways. In this study, we determined whether GABABR are expressed on vagal afferent neurones and whether they modulate distension-evoked discharge of vagal afferents in the isolated stomach. Vagal mehanoreceptor activity was recorded following distensions of the isolated ferret proximal stomach before and after perfusion with the GABABR-selective agonists baclofen and 3-aminopropylphosphinic acid (3-APPiA). Retrograde labeling and immunohistochemistry were used to identify GABABR located on vagal afferent neurones in the nodose ganglia. Vagal afferent fibers responded to isovolumetric gastric distension with an increase in discharge. The GABAB-receptor agonists baclofen (5 × 10-5 M) and 3-APPiA (10-6 to 10-5 M) but not muscimol (GABAA-selective agonist: 1.3 × 10-5 M) significantly decreased afferent distension-response curves. The effect of baclofen (5 × 10-5 M) was reversed by the GABAB-receptor antagonist CGP 62349 (10-5 M). Over 93% of retrogradely labeled gastric vagal afferents in the nodose ganglia expressed immunoreactivity for the GABABR. GABABR expressed on vagal afferent fibers directly inhibit gastric mechanosensory activity. This is likely a contributing mechanism to the efficacy of GABAB-receptor agonists in reducing TLESR and reflux episodes in vivo.

gastric mechanoreceptors; vagus nerve


This article has been cited by other articles:


Home page
 J. Physiol.Home page
V. Snitsarev, C. A. Whiteis, M. W. Chapleau, and F. M. Abboud
Mechano- and chemosensitivity of rat nodose neurones - selective excitatory effects of prostacyclin
J. Physiol., July 1, 2007; 582(1): 177 - 194.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. L. Young, A. J. Page, T. A. O'Donnell, N. J. Cooper, and L. A. Blackshaw
Peripheral versus central modulation of gastric vagal pathways by metabotropic glutamate receptor 5
Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G501 - G511.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
G. Burdyga, A. Varro, R. Dimaline, D. G. Thompson, and G. J. Dockray
Ghrelin receptors in rat and human nodose ganglia: putative role in regulating CB-1 and MCH receptor abundance
Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1289 - G1297.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. J Page, J. A Slattery, T. A O'Donnell, N. J Cooper, R. L Young, and L. A. Blackshaw
Modulation of gastro-oesophageal vagal afferents by galanin in mouse and ferret
J. Physiol., March 15, 2005; 563(3): 809 - 819.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. N. Sengupta, B. K. Medda, and R. Shaker
Effect of GABAB receptor agonist on distension-sensitive pelvic nerve afferent fibers innervating rat colon
Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1343 - G1351.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online