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Am J Physiol Gastrointest Liver Physiol 282: G137-G144, 2002; doi:10.1152/ajpgi.00057.2002
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Vol. 282, Issue 1, G137-G144, January 2002

ATP-sensitive K+ channels composed of Kir6.1 and SUR2B subunits in guinea pig gastric myocytes

Jae Hoon Sim1, Dong Ki Yang1, Young Chul Kim2, Sung Jin Park1, Tong Mook Kang3, Insuk So1, and Ki Whan Kim1

1 Department of Physiology and Biophysics, Seoul National University College of Medicine, Seoul 110-799; 2 Department of Physiology, College of Medicine, Chosun University, Kwangju 501-759; and 3 Department of Physiology, Sungkyunkwan University College of Medicine, Suwon 440-746, Korea

This study was designed to identify the single-channel properties and molecular entity of ATP-sensitive K+ (KATP) channels in guinea pig gastric myocytes with patch-clamp recording and RT-PCR. Pinacidil and diazoxide activated KATP currents in a glibenclamide-sensitive manner. The open probability of channels was enhanced by the application of 10 µM pinacidil from 0.085 ± 0.04 to 0.20 ± 0.05 (n = 7) and was completely blocked by 10 µM glibenclamide. Single-channel conductance was 37.3 ± 2.5 pS (n = 4) between -80 and -20 mV in symmetrical K+ gradient conditions. In inside-out mode, KATP channels showed no spontaneous openings and were activated by the application of nucleotide diphosphates to the cytoplasmic side. These single-channel properties are similar to those of the nucleotide diphosphate-dependent K+ channels in vascular smooth muscle, which are composed of Kir6.1 and sulfonylurea receptor (SUR)2B. RT-PCR demonstrated the presence of Kir6.1, Kir6.2, and SUR2B in guinea pig stomach smooth muscle cells. These results suggest that KATP channels in smooth muscle cells of the guinea pig stomach are composed of Kir6.1 and SUR2B.

adenosine 5'-triphosphate-sensitive potassium channels; Kir6.1-sulfonurea receptor 2B; smooth muscle cells; guinea pig stomach


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 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Y. M. Lee, B. J. Kim, H. J. Kim, D. K. Yang, M. H. Zhu, K. P. Lee, I. So, and K. W. Kim
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Am J Physiol Gastrointest Liver Physiol, April 1, 2003; 284(4): G604 - G616.
[Abstract] [Full Text] [PDF]


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