AJP - GI Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 282: G165-G174, 2002. First published September 21, 2001; doi:10.1152/ajpgi.00326.2001
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/1/G165    most recent
00326.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pozo, M. J.
Right arrow Articles by Mawe, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pozo, M. J.
Right arrow Articles by Mawe, G. M.
Vol. 282, Issue 1, G165-G174, January 2002

Ca2+ sparks and BK currents in gallbladder myocytes: role in CCK-induced response

María J. Pozo1, Guillermo J. Pérez2, Mark T. Nelson2, and Gary M. Mawe2,3

1 Department of Physiology, University of Extremadura, 10071 Cáceres, Spain; and 2 Department of Pharmacology and 3 Department of Anatomy and Neurobiology, University of Vermont, Burlington, Vermont 05405

We sought to elucidate the regulation of gallbladder smooth muscle (GBSM) excitability by localized Ca2+ release events (sparks) and large-conductance Ca2+-dependent (BK) channels by determining whether sparks exist in GBSM and, if so, whether they activate BK channels. Sparks were identified in isolated GBSM loaded with fluo 4. Each spark was associated with a transient outward current, suggesting communication of ryanodine receptor (RyR) channels with BK channels. This was confirmed by the inhibition of outward currents with iberiotoxin (100 nM), thapsigargin (200 nM), and ryanodine (10 µM). In current clamp mode, the transient BK currents were associated with brief membrane hyperpolarizations (10.9 ± 1.3 mV). Because transient BK currents could dampen GBSM excitability, we tested whether CCK attenuates these events. CCK (10 nM) reduced the amplitude and frequency of transient BK currents, and subsequent caffeine application restored transient BK current activity. These results support the concept that RyRs and BK channels contribute to the regulation of GBSM excitability and that CCK can act in part by inhibiting this pathway.

guinea pig; smooth muscle; sarcoplasmic reticulum; ryanodine receptor; thapsigargin; L-type calcium channels; caffeine


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
O. B. Balemba, A. C. Bartoo, M. T. Nelson, and G. M. Mawe
Role of mitochondria in spontaneous rhythmic activity and intracellular calcium waves in the guinea pig gallbladder smooth muscle
Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G467 - G476.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. A. Williams and S. M. Sims
Calcium sparks activate calcium-dependent Cl current in rat corpus cavernosum smooth muscle cells
Am J Physiol Cell Physiol, October 1, 2007; 293(4): C1239 - C1251.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Morales, A. Diez, A. Puyet, P. J. Camello, C. Camello-Almaraz, J. M. Bautista, and M. J. Pozo
Calcium controls smooth muscle TRPC gene transcription via the CaMK/calcineurin-dependent pathways
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C553 - C563.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Camello-Almaraz, P. J. Gomez-Pinilla, M. J. Pozo, and P. J. Camello
Mitochondrial reactive oxygen species and Ca2+ signaling
Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1082 - C1088.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
O. B. Balemba, T. J. Heppner, A. D. Bonev, M. T. Nelson, and G. M. Mawe
Calcium waves in intact guinea pig gallbladder smooth muscle cells
Am J Physiol Gastrointest Liver Physiol, October 1, 2006; 291(4): G717 - G727.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
O. B. Balemba, M. J. Salter, T. J. Heppner, A. D. Bonev, M. T. Nelson, and G. M. Mawe
Spontaneous electrical rhythmicity and the role of the sarcoplasmic reticulum in the excitability of guinea pig gallbladder smooth muscle cells
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G655 - G664.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. J. Gomez-Pinilla, S. Morales, C. Camello-Almaraz, R. Moreno, M. J. Pozo, and P. J. Camello
Changes in guinea pig gallbladder smooth muscle Ca2+ homeostasis by acute acalculous cholecystitis
Am J Physiol Gastrointest Liver Physiol, January 1, 2006; 290(1): G14 - G22.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Morales, P. J. Camello, G. M. Mawe, and M. J. Pozo
Characterization of intracellular Ca2+ stores in gallbladder smooth muscle
Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G507 - G513.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Morales, P. J. Camello, S. Alcon, G. M. Salido, G. Mawe, and M. J. Pozo
Coactivation of capacitative calcium entry and L-type calcium channels in guinea pig gallbladder
Am J Physiol Gastrointest Liver Physiol, June 1, 2004; 286(6): G1090 - G1100.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online