AJP - GI AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 282: G23-G33, 2002; doi:10.1152/ajpgi.00277.2002
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ammar, D. A.
Right arrow Articles by Yao, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ammar, D. A.
Right arrow Articles by Yao, X.
Vol. 282, Issue 1, G23-G33, January 2002

Syntaxin 3 is required for cAMP-induced acid secretion: streptolysin O-permeabilized gastric gland model

David A. Ammar, Rihong Zhou, John G. Forte, and Xuebiao Yao

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720

Gastric gland stimulation triggers H+,K+-ATPase translocation from cytoplasmic tubulovesicles to apical plasma membrane in parietal cells, resulting in HCl secretion. We studied the mechanisms involved in tubulovesicle translocation with a permeabilized gland system. Streptolysin O (SLO)-treated glands were permeabilized such that exogenous fluorescently labeled actin incorporated into cytoskeleton in a pattern mimicking endogenous F-actin. As shown by accumulation of the weak base aminopyrine (AP), SLO-permeabilized glands are stimulated to secrete acid by addition of cAMP and ATP and inhibited by proton pump inhibitors. Direct visualization with the fluorescent pH probe Lysosensor showed acid accumulation in glandular lumen and parietal cell canaliculi. ME-3407, an antiulcer drug with inhibitory action implicated to involve ezrin, inhibited AP uptake in and effectively released ezrin from intact and SLO-permeabilized glands. In contrast, wortmannin, an effective secretion inhibitor in intact glands, had minimal effects on ezrin or AP accumulation in SLO-permeabilized glands. The finding that SNARE protein syntaxin 3 is associated with H+,K+-ATPase-containing tubulovesicles suggested that it is involved in membrane fusion. Addition of recombinant syntaxin 3, but not syntaxin 5 or heat-denatured syntaxin 3, dose-dependently inhibited acid secretion. Our studies are consistent with a membrane recycling hypothesis that activation of protein kinase cascades leads to SNARE-mediated fusion of H+,K+-ATPase-containing tubulovesicles to apical plasma membrane.

SNARE hypothesis; membrane fusion; ezrin; wortmannin; hydrogen,potassium-adenosinetriphosphatase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
P. A. Ortiz
cAMP increases surface expression of NKCC2 in rat thick ascending limbs: role of VAMP
Am J Physiol Renal Physiol, March 1, 2006; 290(3): F608 - F616.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
N. McDaniel, A. J. Pace, S. Spiegel, R. Engelhardt, B. H. Koller, U. Seidler, and C. Lytle
Role of Na-K-2Cl cotransporter-1 in gastric secretion of nonacidic fluid and pepsinogen
Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G550 - G560.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Zhou, C. Watson, C. Fu, X. Yao, and J. G. Forte
Myosin II is present in gastric parietal cells and required for lamellipodial dynamics associated with cell activation
Am J Physiol Cell Physiol, September 1, 2003; 285(3): C662 - C673.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. G. Duman and J. G. Forte
What is the role of SNARE proteins in membrane fusion?
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C237 - C249.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Zhou, Z. Guo, C. Watson, E. Chen, R. Kong, W. Wang, and X. Yao
Polarized Distribution of IQGAP Proteins in Gastric Parietal Cells and Their Roles in Regulated Epithelial Cell Secretion
Mol. Biol. Cell, March 1, 2003; 14(3): 1097 - 1108.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online