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Division of Physiology, Department of Medical Cell Biology, University of Uppsala, 751 23 Uppsala, Sweden
Mucus thickness is suggested to be related to mucosal protection. We therefore investigated the importance of the removable mucous layer and epithelial bicarbonate transport in preservation of the gastric juxtamucosal pH (pHjm) during luminal acid. Anesthetized rats were prepared for intravital microscopy of the gastric mucosa, and pHjm was measured with pH-sensitive microelectrodes. The mucus was either left intact (IM) or removed (MR) down to the firmly attached mucous layer, and HCl (pH 1) was applied luminally. Removal of the loosely adherent mucous layer did not influence the pHjm during luminal acid (pentagastrin: IM/MR 7.03 ± 0.09/6.82 ± 0.19; pentagastrin + indomethacin: IM/MR 6.89 ± 0.20/6.95 ± 0.27; ranitidine: IM/MR 2.38 ± 0.64/2.97 ± 0.62), unless prostaglandin synthesis and acid secretion were inhibited (ranitidine + indomethacin: IM/MR 2.03 ± 0.37/1.66 ± 0.18). Neutral pHjm is maintained during endogenous acid secretion and luminal pH 1, unless DIDS was applied luminally, which resulted in a substantially decreased pHjm (1.37 ± 0.21). Neutral pHjm is maintained by a DIDS-sensitive bicarbonate transport over the surface epithelium. The loosely adherent mucous layer only contributes to maintaining pHjm during luminal pH 1 if acid secretion and prostaglandin synthesis are inhibited.
pH-sensitive microelectrodes; 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid; rat
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