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Am J Physiol Gastrointest Liver Physiol 282: G324-G331, 2002; doi:10.1152/ajpgi.00198.2001
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Vol. 282, Issue 2, G324-G331, February 2002

Aquaporins in rat pancreatic interlobular ducts

Shigeru B. H. Ko1, Satoru Naruse1, Motoji Kitagawa1, Hiroshi Ishiguro1, Sonoko Furuya2, Nobumasa Mizuno1, Youxue Wang1, Toshiyuki Yoshikawa1, Atsushi Suzuki1, Shoko Shimano1, and Tetsuo Hayakawa1

1 Internal Medicine II, Nagoya University School of Medicine, Showa-ku, Nagoya 466-8550, and 2 National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, Japan

The aquaporin (AQP) family of water channels is distributed ubiquitously in many epithelia and plays a fundamental role in transmembrane water transport. The aim of this study is to identify the water transport pathway in pancreatic duct cells where most of the HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>-rich fluid originates. Using digital videomicroscopy, we measured the osmotic water permeability (Pf) of pancreatic duct epithelium by exposing isolated rat interlobular ducts to the hypotonic solution (145 mosM). To identify mRNA and protein of AQPs expressed in duct cells, we conducted RT-PCR analysis and immunohistochemistry of the isolated duct and pancreas. The calculated Pf (160-230 µm/s) of the isolated ducts was significantly reduced to 16-35 µm/s by 80-90% with either basolateral or luminal applications of HgCl2. Fluid secretion evoked by secretin was almost completely abolished by a basolateral or luminal application of HgCl2. A large amount of AQP1 and small amounts of AQP5 transcripts were detected in the isolated duct cells by RT-PCR. AQP1, but not AQP5, immunoreactivity was present in both luminal and basolateral membranes of the interlobular duct cells. Mercury-sensitive water channels are present in both luminal and basolateral membranes of rat pancreatic ducts. AQP1 of the known AQPs appears to be the main water pathway in interlobular ducts.

isolated pancreatic duct; mercury-sensitive water channel; water permeability; fluid secretion


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