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Am J Physiol Gastrointest Liver Physiol 282: G397-G402, 2002; doi:10.1152/ajpgi.00519.2001
0193-1857/02 $5.00
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Vol. 282, Issue 3, G397-G402, March 2002

THEME
Cutting-edge technology
I. Global gene expression profiling using DNA microarrays

Mayi Arcellana-Panlilio and Stephen M. Robbins

Southern Alberta Microarray Facility, Departments of Oncology and Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada

Having the complete human genomic sequence poses a new challenge: to use genomic structural information to display and analyze biological processes on a genome-wide scale to assign gene function. DNA microarrays are a miniaturized, ordered arrangement of nucleic acid fragments from individual genes located at defined positions on a solid support, enabling the analysis of thousands of genes in parallel by specific hybridization. This review describes technical aspects, discusses relevant applications, and suggests factors affecting the use of this technology and how it fits in the grand scheme of meeting the needs of the postgenomic era.

genome; human; gene expression profiling; oligonucleotide array sequence analysis; genetic techniques


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