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Am J Physiol Gastrointest Liver Physiol 282: G480-G490, 2002; doi:10.1152/ajpgi.00531.2000
0193-1857/02 $5.00
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Vol. 282, Issue 3, G480-G490, March 2002

Region-specific ontogeny of alpha -2,6-sialyltransferase during normal and cortisone-induced maturation in mouse intestine

Dingwei Dai1,2, N. Nanda Nanthakumar2, Tor C. Savidge2, David S. Newburg3, and W. Allan Walker2

1 Shanghai Institute for Pediatric Research, Xinhua Hospital and Shanghai Second Medical University, Shanghai, People's Republic of China 200092; 2 Developmental Gastroenterology Laboratory, Combined Program in Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital and Harvard Medical School, Charlestown 02129; and 3 Program in Glycobiology, Shriver Center for Mental Retardation, Waltham, Massachusetts 02452

Regional differences in the ontogeny of mouse intestinal alpha -2,6-sialyltransferase activities (alpha -2,6-ST) and the influence of cortisone acetate (CA) on this expression were determined. High ST activity and alpha -2,6-ST mRNA levels were detected in immature small and large intestine, with activity increasing distally from the duodenum. As the mice matured, ST activity (predominantly alpha -2,6-ST) in the small intestine decreased rapidly to adult levels by the fourth postnatal week. CA precociously accelerated this region-specific ontogenic decline. A similar decline of ST mRNA levels reflected ST activity in the small, but not the large, intestine. Small intestinal sialyl alpha -2,6-linked glycoconjugates displayed similar developmental and CA induced-precocious declines when probed using Sambucus nigra agglutinin (SNA) lectin. SNA labeling demonstrated age-dependent diminished sialyl alpha 2,6 glycoconjugate expression in goblet cells in the small (but not large) intestine, but no such regional specificity was apparent in microvillus membrane. This suggests differential regulation of sialyl alpha -2,6 glycoconjugates in absorptive vs. globlet cells. These age-dependent and region-specific differences in sialyl alpha -2,6 glycoconjugates may be mediated in part by altered alpha -2,6-ST gene expression regulated by trophic factors such as glucocorticoids.

sialyl alpha -2,6 glycoconjugates; hormonal regulation; ontogeny of the gut; lectin


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