AJP - GI Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 282: G508-G518, 2002. First published November 21, 2001; doi:10.1152/ajpgi.00291.2001
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/3/G508    most recent
00291.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ko, W. H.
Right arrow Articles by Huang, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ko, W. H.
Right arrow Articles by Huang, Y.
Vol. 282, Issue 3, G508-G518, March 2002

Stimulation of chloride secretion by baicalein in isolated rat distal colon

W. H. Ko1, V. W. Y. Law1, W. C. Y. Yip1, G. G. L. Yue1, C. W. Lau1, Z. Y. Chen2, and Y. Huang1

1 Department of Physiology and 2 Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong, China

The effect of baicalein on mucosal ion transport in the rat distal colon was investigated in Ussing chambers. Mucosal addition of baicalein (1-100 µM) elicited a concentration-dependent short-circuit current (Isc) response. The increase in Isc was mainly due to Cl- secretion. The presence of mucosal indomethacin (10 µM) significantly reduced both the basal and subsequent baicalein-evoked Isc responses. The baicalein-induced Isc were inhibited by mucosal application of diphenylamine-2-carboxylic acid (100 µM) and glibenclamide (500 µM) and basolateral application of chromanol 293B (30 µM), a blocker of KvLQT1 channels and Ba2+ ions (5 mM). Treatment of the colonic mucosa with baicalein elicited a threefold increase in cAMP production. Pretreating the colonic mucosa with carbachol (100 µM, serosal) but not thapsigargin (1 µM, both sides) abolished the baicalein-induced Isc. Addition of baicalein subsequent to forskolin induced a further increase in Isc. These results indicate that the baicalein evoked Cl- secretion across rat colonic mucosa, possibly via a cAMP-dependent pathway. However, the action of baicalein cannot be solely explained by its cAMP-elevating effect. Baicalein may stimulate Cl- secretion via a cAMP-independent pathway or have a direct effect on cystic fibrosis transmembrane conductance regulator.

Ussing chambers; isolated crypts; intracellular cyclic adenosine 5'-monophosphate; colonic mucosa


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
D. Wu and Z. Hu
Rutaecarpine Induces Chloride Secretion across Rat Isolated Distal Colon
J. Pharmacol. Exp. Ther., April 1, 2008; 325(1): 256 - 266.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
I. Blumenstein, B. Keseru, F. Wolter, and J. Stein
The Chemopreventive Agent Resveratrol Stimulates Cyclic AMP-Dependent Chloride Secretion In vitro
Clin. Cancer Res., August 1, 2005; 11(15): 5651 - 5656.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P. Cavelier and D. Attwell
Tonic release of glutamate by a DIDS-sensitive mechanism in rat hippocampal slices
J. Physiol., April 15, 2005; 564(2): 397 - 410.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online