AJP - GI AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 282: G540-G548, 2002. First published November 28, 2001; doi:10.1152/ajpgi.00405.2001
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/3/G540    most recent
00405.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zinchuk, V. S.
Right arrow Articles by Seguchi, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zinchuk, V. S.
Right arrow Articles by Seguchi, H.
Vol. 282, Issue 3, G540-G548, March 2002

Asynchronous expression and colocalization of Bsep and Mrp2 during development of rat liver

Vadim S. Zinchuk1, Teruhiko Okada1, Kunihiro Akimaru2, and Harumichi Seguchi1

1 Department of Anatomy and Cell Biology and 2 Department of Environmental and Occupational Health, Kochi Medical School, Nankoku, Kochi 783-8505, Japan

In the liver, function of the bile salt export pump (Bsep), a major canalicular exporter of bile salts, is complemented by activity of the multidrug resistance protein 2 (Mrp2), a canalicular organic anions transporter. Mrp2 was found capable of transporting various anticancer drugs out of cells, eventually undermining their therapeutic potential and contributing to multidrug resistance. We employed a RT-PCR, immunoblotting, and immunofluorescence to examine their gene, protein expression, and distribution of antigenic sites in the rat liver during development from 16-day-old fetus to adult animal. Bsep mRNA was almost undetectable before birth. It was first clearly expressed in the liver of newborn rats. On the contrary, Mrp2 mRNA was seen before birth, although at low levels. In concert with mRNA expression, Bsep protein was undetectable before birth, while Mrp2 protein was already expressed. Both proteins were clearly detectable in the postnatal period. Confocal immunofluorescent microscopy showed asynchronous appearance of Bsep and Mrp2 proteins during development but their colocalization in the bile canaliculi once each one is expressed. During the gestational period, a weak immunofluorescence for Mrp2 was observed only in livers of 16-day-old embryos. No fluorescence for Bsep was seen. Both proteins were clearly visualizable after birth, although the pattern of immunostaining varied. These findings provide molecular evidence that expression of both Bsep and Mrp2 during development is transcriptionally regulated. They also point out the differences in relevance to the liver function of the systems responsible for canalicular transport of bile salts versus organic anions.

canalicular transporters; rat; ATP-binding cassette proteins; ontogenesis


This article has been cited by other articles:


Home page
 Drug Metab. Dispos.Home page
N. Li, Y. Zhang, F. Hua, and Y. Lai
Absolute Difference of Hepatobiliary Transporter Multidrug Resistance-Associated Protein (MRP2/Mrp2) in Liver Tissues and Isolated Hepatocytes from Rat, Dog, Monkey, and Human
Drug Metab. Dispos., January 1, 2009; 37(1): 66 - 73.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. V. St-Pierre, T. Stallmach, A. Freimoser Grundschober, J.-F. Dufour, M. A. Serrano, J. J. G. Marin, Y. Sugiyama, and P. J. Meier
Temporal expression profiles of organic anion transport proteins in placenta and fetal liver of the rat
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2004; 287(6): R1505 - R1516.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
H A Lemonde, E J Custard, J Bouquet, M Duran, H Overmars, P J Scambler, and P T Clayton
Mutations in SRD5B1 (AKR1D1), the gene encoding {Delta}4-3-oxosteroid 5{beta}-reductase, in hepatitis and liver failure in infancy
Gut, October 1, 2003; 52(10): 1494 - 1499.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. Trauner and J. L. Boyer
Bile Salt Transporters: Molecular Characterization, Function, and Regulation
Physiol Rev, April 1, 2003; 83(2): 633 - 671.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online