AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 282: G565-G572, 2002; doi:10.1152/ajpgi.00512.2000
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, Q.
Right arrow Articles by Rockey, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, Q.
Right arrow Articles by Rockey, D. C.
Vol. 282, Issue 3, G565-G572, March 2002

Adenovirus-mediated gene transfer to nonparenchymal cells in normal and injured liver

Qing Yu1,2, Loretta G. Que1, and Don C. Rockey1,2

1 Departments of Medicine and 2 Cell Biology, Duke University Medical Center, Durham, North Carolina 27710

Adenovirus-mediated gene transfer has become an important tool with which to introduce genetic material into cells. Available data emphasize efficient adenoviral transduction of parenchymal liver cells (i.e., hepatocytes) in both in vitro and in vivo model systems, typically in normal cells. The aim of this study was to evaluate gene transfer to nonparenchymal (and parenchymal) cells of the normal and injured rat liver. Hepatocytes, stellate cells, and endothelial cells were isolated by standard methods. Liver injury was induced by bile duct ligation or carbon tetrachloride administration. Cells were transduced in vitro with an adenovirus encoding beta -galactosidase (Ad.beta -gal) over a range of viral titers, and transduced cells were identified by detection of X-gal. In vivo transduction efficiency was studied in normal and injured livers using cell isolation techniques. Nonparenchymal cells were transduced with greater frequency than hepatocytes at all adenoviral titers tested, both in vitro and in vivo. After liver injury, adenoviral transduction was reduced for all liver cell types compared with that for cells from normal livers (at all virus titers). Notably, transduction efficiency remained greater in nonparenchymal cells than in hepatocytes after liver injury. This work implies that, to achieve comparable gene expression in the injured liver, higher adenoviral titers may be required, an important consideration as gene therapy in disease states is considered.

cirrhosis; bile duct ligation; carbon tetrachloride; stellate cell; sinusoidal endothelial cell


This article has been cited by other articles:


Home page
 GutHome page
K. Kinoshita, Y. Iimuro, J. Fujimoto, Y. Inagaki, K. Namikawa, H. Kiyama, Y. Nakajima, K. Otogawa, N. Kawada, S. L Friedman, et al.
Targeted and regulable expression of transgenes in hepatic stellate cells and myofibroblasts in culture and in vivo using an adenoviral Cre/loxP system to antagonise hepatic fibrosis
Gut, March 1, 2007; 56(3): 396 - 404.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Yang, C.-C. Chan, O.-S. Kwon, S. Liu, J. McGhee, S. A. Stimpson, L. Z. Chen, W. W. Harrington, W. T. Symonds, and D. C. Rockey
Regulation of peroxisome proliferator-activated receptor-{gamma} in liver fibrosis
Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G902 - G911.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. Vincent, R. F. Pettersson, R. G. Crystal, and P. L. Leopold
Cytokine-Mediated Downregulation of Coxsackievirus-Adenovirus Receptor in Endothelial Cells
J. Virol., August 1, 2004; 78(15): 8047 - 8058.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H. Hendrickson, S. Chatterjee, S. Cao, M. M. Ruiz, W. C. Sessa, and V. Shah
Influence of caveolin on constitutively activated recombinant eNOS: insights into eNOS dysfunction in BDL rat liver
Am J Physiol Gastrointest Liver Physiol, August 8, 2003; 285(3): G652 - G660.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online