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1 Department of Gastroenterology, University of Heidelberg, 69115 Heidelberg, Germany; and 2 Graduate School of Pharmaceutical Sciences, The University of Tokyo, 113 - 0033 Tokyo, Japan
Multidrug
resistance-associated protein 3 (MRP3; symbol ABCC3), has been shown to
mediate ATP-dependent transport of organic anions including
17
-glucuronosyl estradiol, glucuronosyl bilirubin, monovalent, and
sulfated bile salts. MRP3 mRNA expression was reported in
rat intestine suggesting a role of MRP3 in the intestinal transport. We
examined the expression and localization of MRP3 in rat small and large
intestine by RT-PCR, immunofluorescence, and immunoblot
analysis. MRP3 was identified in all intestinal segments by
RT-PCR. MRP3 expression was low in duodenum and jejunum but markedly
increased in ileum and colon. With the use of a rat MRP3 specific
antibody, MRP3 was localized to the basolateral domains of enterocytes.
Immunofluorescence analysis and immunoblot analysis confirmed a strong
expression of rat MRP3 in ileum and colon. In contrast, MRP2 was
predominantly expressed in the proximal segments of rat small
intestine. Our findings demonstrate a high expression of rat MRP3 in
ileum and colon and provide evidence for an involvement of MRP3 in the
ATP-dependent transport of organic anions, including bile salts from
the enterocyte into blood.
enterohepatic circulation; intestinal transport; organic anions; bile acids
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