AJP - GI Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 282: G1079-G1087, 2002. First published January 16, 2002; doi:10.1152/ajpgi.00041.2001
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/6/G1079    most recent
00041.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, L.
Right arrow Articles by Li, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, L.
Right arrow Articles by Li, E.
Vol. 282, Issue 6, G1079-G1087, June 2002

Analysis of human cellular retinol-binding protein II promoter during enterocyte differentiation

Liang Zhang1, Xueping E1, Kathryn E. Luker1, Jian-Su Shao1, Marc S. Levin1,3, Eunran Suh4, and Ellen Li1,2

Departments of 1 Medicine and 2 Biochemistry and Molecular Biophysics, Washington University- St. Louis School of Medicine and 3 Specialty Care, St. Louis Veterans Administration Medical Center, St. Louis, Missouri 63110; and 4 Department of Internal Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104

Cellular retinol binding protein II (CRBP II) is a vitamin A-binding protein that is expressed specifically in small intestinal villus absorptive cells. Previous studies have shown that retinoic acid upregulates endogenous human CRBP II gene expression in differentiated Caco-2 cells. To better characterize the regulation of human CRBP II expression, we analyzed the ability of receptor-selective agonists to enhance transcription from the 5'-upstream flanking region of the human CRBP II gene. Stable transfection experiments showed that the proximal 2.8-kb region of the human CRBP II gene is sufficient for retinoic acid inducibility in differentiated Caco-2 cells. However, direct sequence analysis and transient transfection experiments indicate that, unlike the rat CRBP II promoter, the human CRBP II promoter is not a direct retinoid X receptor target. The results indicate that the retinoic acid responsiveness of the human CRBP II promoter is mediated by an indirect mechanism and that this mechanism is associated with enterocyte differentiation.

gene regulation; retinoid X receptor; vitamin A; Caco-2 cells


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
N. Yamaguchi, S. Miyamoto, Y. Ogura, T. Goda, and K. Suruga
Hepatocyte nuclear factor-4{alpha} regulates human cellular retinol-binding protein type II gene expression in intestinal cells
Am J Physiol Gastrointest Liver Physiol, March 1, 2009; 296(3): G524 - G533.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online