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Am J Physiol Gastrointest Liver Physiol 282: G1097-G1104, 2002. First published February 20, 2002; doi:10.1152/ajpgi.00320.2001
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Vol. 282, Issue 6, G1097-G1104, June 2002

Intestinal inflammation modulates expression of the synaptic vesicle protein neuronal calcium sensor-1

S. Lourenssen1, A. Jeromin2, J. Roder2, and M. G. Blennerhassett1

1 Gastrointestinal Diseases Research Unit, Queens University, Kingston K7L 5G2; and 2 Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5

The calcium-binding protein neuronal calcium sensor 1 (NCS-1) is involved in modulation of neurotransmitter release in the peripheral and central nervous systems. Since intestinal inflammation impairs neurotransmitter release, we evaluated the expression of NCS-1 in the normal rat colon and in dinitrobenzene sulfonic acid (DNBS)-induced colitis. Immunocytochemistry and Western blots showed high levels of NCS-1 in the myenteric plexus and in axons in the smooth muscle layers; 23 ± 2% of myenteric neurons were NCS-1 positive, with staining restricted to the largest neurons. NCS-1-positive axons decreased to 13.3 ± 0.4% of total axons by day 2 and dropped further to 7.0 ± 0.1% by day 4, returning to control levels by day 16. Dual-label Western blot analysis showed that the expression of NCS-1 relative to PGP 9.5 decreased by 50% on day 4 but returned to control by day 16. The selective loss of NCS-1 during colitis may underlie the altered neural function seen in the inflamed intestine.

frequenin; enteric nervous system; dinitrobenzene sulfonic acid


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