AJP - GI Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 282: G1105-G1112, 2002; doi:10.1152/ajpgi.00431.2001
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (33)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Demols, A.
Right arrow Articles by Deviere, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Demols, A.
Right arrow Articles by Deviere, J.
Vol. 282, Issue 6, G1105-G1112, June 2002

Endogenous interleukin-10 modulates fibrosis and regeneration in experimental chronic pancreatitis

Anne Demols1,2, Jean-Luc Van Laethem1,2, Eric Quertinmont2, Chantal Degraef3, Myriam Delhaye1,2, Albert Geerts4, and Jacques Deviere1,2

1 Department of Gastroenterology, 2 Hôpital Erasme and Laboratory of Experimental Gastroenterology, and 3 Laboratory of Experimental Cytology and Cancerology, Université Libre de Bruxelles, B 1070 Brussels, Belgium; and 4 Department of Medical Cell Biology, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE2 4HH, United Kingdom

Interleukin (IL)-10, a potent anti-inflammatory cytokine, limits the severity of acute pancreatitis and downregulates transforming growth factor (TGF)-beta release by inflammatory cells on stimulation. Proinflammatory mediators, reactive oxygen species, and TGF-beta can activate pancreatic stellate cells and their synthesis of collagen I and III. This study evaluates the role of endogenous IL-10 in the modulation of the regeneration phase following acute pancreatitis and in the development of pancreatic fibrosis. IL-10 knockout (KO) mice and their C57BL/6 controls were submitted to repeated courses (3/wk, during 6 wk, followed by 1 wk of recovery) of cerulein-induced acute pancreatitis. TGF-beta 1 release was measured on plasma, and its pancreatic expression was assessed by quantitative RT-PCR and immunohistochemistry. Intrapancreatic IL-10 gene expression was assessed by semiquantitative RT-PCR, and intrapancreatic collagen content was assessed by picrosirius staining. Activated stellate cells were detected by immunohistochemistry. S phase intrapancreatic cells were marked using tritiated thymidine labeling. After repeated acute pancreatitis, IL-10 KO mice had more severe histological lesions and fibrosis (intrapancreatic collagen content) than controls. TGF-beta 1 plasma levels, intrapancreatic transcription, and expression by ductal and interstitial cells, as well as the number of activated stellate cells, were significantly higher. IL-10 KO mice disclosed significantly fewer acinar cells in S phase, whereas the opposite was observed for pseudotubular cells. Endogenous IL-10 controls the regeneration phase and limits the severity of fibrosis and glandular atrophy induced by repeated episodes of acute pancreatitis in mice.

experimental chronic pancreatitis; pancreatic fibrosis; transforming growth factor-beta ; pancreatic stellate cells


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
I. Gukovsky, A. Lugea, M. Shahsahebi, J. H. Cheng, P. P. Hong, Y. J. Jung, Q.-g. Deng, B. A. French, W. Lungo, S. W. French, et al.
A rat model reproducing key pathological responses of alcoholic chronic pancreatitis
Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G68 - G79.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J.-A. Gomez, X. Molero, E. Vaquero, A. Alonso, A. Salas, and J.-R. Malagelada
Vitamin E attenuates biochemical and morphological features associated with development of chronic pancreatitis
Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G162 - G169.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
D. A. Horwitz, S. G. Zheng, and J. D. Gray
The role of the combination of IL-2 and TGF-{beta} or IL-10 in the generation and function of CD4+ CD25+ and CD8+regulatory T cell subsets
J. Leukoc. Biol., October 1, 2003; 74(4): 471 - 478.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online