AJP - GI Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 283: G809-G818, 2002. First published April 24, 2002; doi:10.1152/ajpgi.00057.2002
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/G809    most recent
00057.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (30)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Simmons, J. G.
Right arrow Articles by Lund, P. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Simmons, J. G.
Right arrow Articles by Lund, P. K.
Vol. 283, Issue 3, G809-G818, September 2002

IGF-I and TGF-beta 1 have distinct effects on phenotype and proliferation of intestinal fibroblasts

James G. Simmons1, Jolanta B. Pucilowska1,2, Temitope O. Keku2, and P. Kay Lund1,2

1 Department of Cell and Molecular Physiology and 2 Center for Gastrointestinal Biology and Disease, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7545

Insulin-like growth factor I (IGF-I) and transforming growth factor-beta 1 (TGF-beta 1) are upregulated in myofibroblasts at sites of fibrosis in experimental enterocolitis and in Crohn's disease (CD). We compared the sites of expression of IGF-I and TGF-beta 1 in a rat peptidoglycan-polysaccharide (PG-PS) model of chronic granulomatous enterocolitis and fibrosis. We used the human colonic CCD-18Co fibroblast/myofibroblast cell line to test the hypothesis that TGF-beta 1 and IGF-I interact to regulate proliferation, collagen synthesis, and activated phenotype typified by expression of alpha -smooth muscle actin and organization into stress fibers. IGF-I potently stimulated while TGF-beta 1 inhibited basal DNA synthesis. TGF-beta 1 and IGF-I each had similar but not additive effects to induce type I collagen. TGF-beta 1 but not IGF-I potently stimulated expression of alpha -smooth muscle actin and stress fiber formation. IGF-I in combination with TGF-beta 1 attenuated stress fiber formation without reducing alpha -smooth muscle actin expression. Stress fibers were not a prerequisite for increased collagen synthesis. TGF-beta 1 upregulated IGF-I mRNA, which led us to examine the effects of IGF-I in cells previously activated by TGF-beta 1 pretreatment. IGF-I potently stimulated proliferation of TGF-beta 1-activated myofibroblasts without reversing activated fibrogenic phenotype. We conclude that TGF-beta 1 and IGF-I both stimulate type I collagen synthesis but have differential effects on activated phenotype and proliferation. We propose that during intestinal inflammation, regulation of activated phenotype and proliferation may require sequential actions of TGF-beta 1 and IGF-I, but they may act in concert to increase collagen deposition.

Crohn's disease; alpha -smooth muscle actin; fibrosis


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. G. Simmons, Y. Ling, H. Wilkins, C. R. Fuller, A. J. D'Ercole, J. Fagin, and P. K. Lund
Cell-specific effects of insulin receptor substrate-1 deficiency on normal and IGF-I-mediated colon growth
Am J Physiol Gastrointest Liver Physiol, November 1, 2007; 293(5): G995 - G1003.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
F Rieder, J Brenmoehl, S Leeb, J Scholmerich, and G Rogler
Wound healing and fibrosis in intestinal disease
Gut, January 1, 2007; 56(1): 130 - 139.
[Full Text] [PDF]

Home page
 Int ImmunolHome page
S. Finotto, M. Hausding, A. Doganci, J. H. Maxeiner, H. A. Lehr, C. Luft, P. R. Galle, and L. H. Glimcher
Asthmatic changes in mice lacking T-bet are mediated by IL-13
Int. Immunol., August 1, 2005; 17(8): 993 - 1007.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
D. W. Powell, P. A. Adegboyega, J. F. Di Mari, and R. C. Mifflin
Epithelial Cells and Their Neighbors I. Role of intestinal myofibroblasts in development, repair, and cancer
Am J Physiol Gastrointest Liver Physiol, July 1, 2005; 289(1): G2 - G7.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Chitapanarux, S. L. Chen, H. Lee, A. C. Melton, and H. F. Yee Jr.
C-type natriuretic peptide induces human colonic myofibroblast relaxation
Am J Physiol Gastrointest Liver Physiol, January 1, 2004; 286(1): G31 - G36.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online