AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 283: G1175-G1184, 2002. First published July 17, 2002; doi:10.1152/ajpgi.00515.2001
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/5/G1175    most recent
00515.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takahashi, Y.
Right arrow Articles by Murase, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takahashi, Y.
Right arrow Articles by Murase, N.
Vol. 283, Issue 5, G1175-G1184, November 2002

Role of NF-kappa B on liver cold ischemia-reperfusion injury

Yoshihito Takahashi1, Raymond W. Ganster1, Andrea Gambotto1, Lifang Shao1, Takashi Kaizu1, Tong Wu2, Gautam P. Yagnik1, Atsunori Nakao1, George Tsoulfas1, Takashi Ishikawa1, Toyokazu Okuda1, David A. Geller1, and Noriko Murase1

Thomas E. Starzl Transplantation Institute, Departments of 1 Surgery and 2 Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213

The role of NF-kappa B, the rapid-response transcription factor for multiple genes, in cold ischemia-reperfusion (I/R) injury was examined after syngeneic transplantation of liver grafts. Lewis rat recipients were killed 1-48 h after reperfusion of three different liver grafts: 1) uninfected control, 2) infected ex vivo with control adenoviral vector (AdEGFP), and 3) infected ex vivo with AdIkappa B. In uninfected control livers, NF-kappa B was activated biphasically at 1-3 and 12 h after reperfusion with aspartate transaminase (AST) levels of 4,244 ± 691 IU/l. The first peak of NF-kappa B activation associated with an increase of mRNA for TNF-alpha , IL-1beta , and IL-10. AdEGFP transfection resulted in similar outcomes. Interestingly, AdIkappa B-transfected liver grafts suffered more severe I/R injury (AST >9,000 IU/l). Transfected Ikappa B was detected in transplanted livers as early as 6 h, and this correlated with the abrogation of the second, but not the first, peak of NF-kappa B activation at 12-48 h and increased apoptosis. Thus inhibition of the second wave of NF-kappa B activation in Ikappa B-transfected livers resulted in an increase of liver injury, suggesting that NF-kappa B may have a dual role during liver I/R injury.

liver transplantation; preservation injuries; Ikappa B; adenoviral vector


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Kaizu, A. Ikeda, A. Nakao, A. Tsung, H. Toyokawa, S. Ueki, D. A. Geller, and N. Murase
Protection of transplant-induced hepatic ischemia/reperfusion injury with carbon monoxide via MEK/ERK1/2 pathway downregulation
Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G236 - G244.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
X. Chen, W.-X. Ding, H.-M. Ni, W. Gao, Y.-H. Shi, A. A. Gambotto, J. Fan, A. A. Beg, and X.-M. Yin
Bid-Independent Mitochondrial Activation in Tumor Necrosis Factor Alpha-Induced Apoptosis and Liver Injury
Mol. Cell. Biol., January 15, 2007; 27(2): 541 - 553.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Tsung, M. T. Stang, A. Ikeda, N. D. Critchlow, K. Izuishi, A. Nakao, M. H. Chan, G. Jeyabalan, J. H. Yim, and D. A. Geller
The transcription factor interferon regulatory factor-1 mediates liver damage during ischemia-reperfusion injury
Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1261 - G1268.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
A. Tsung, R. Sahai, H. Tanaka, A. Nakao, M. P. Fink, M. T. Lotze, H. Yang, J. Li, K. J. Tracey, D. A. Geller, et al.
The nuclear factor HMGB1 mediates hepatic injury after murine liver ischemia-reperfusion
J. Exp. Med., April 4, 2005; 201(7): 1135 - 1143.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online