| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Anatomy and Neurobiology, University of Vermont College of Medicine, Burlington, Vermont, 05405; and 2 Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada T2N 4N1
PGE2 is a proinflammatory mediator that can influence many cell types. This study was conducted to determine whether PGE2 alters the electrical activity of distal colonic myenteric neurons, because colitis is typically associated with altered motility and changes in neural signaling may be involved. The electrical properties of intact myenteric neurons were evaluated with intracellular microelectrodes. Acute application of PGE2 elicited a prolonged depolarization in both AH and S neurons with little effect on input resistance or electrical excitability. PGE2 effects were suppressed by tetrodotoxin (TTX) or neurokinin (NK) receptor antagonists, indicating that PGE2 acts directly and indirectly to depolarize colonic neurons. PGE2-evoked depolarization was concentration dependent (~3 µM EC50) and was attenuated by the E prostanoid (EP)1/2 receptor antagonist, AH-6809. When preparations were maintained for 48 h in the presence of the stable PGE2 analog PGE2-ethanolamide (10 µM), neurons exhibited a significant membrane depolarization and enhanced excitability. These results suggest that PGE2 can play a role in altered motility in colitis by evoking changes in the electrical properties of myenteric neurons.
motility; inflammation; colitis; innervation; enteric nervous system
This article has been cited by other articles:
|
|
 |
|
  J. R O'Hara, A. E Lomax, G. M Mawe, and K. A Sharkey Ileitis alters neuronal and enteroendocrine signalling in guinea pig distal colon Gut, February 1, 2007; 56(2): 186 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rehn, D. Hild, and M. Diener Upregulation of cyclooxygenase-2 and thromboxane A2 production mediate the action of tumor necrosis factor-{alpha} in isolated rat myenteric ganglia Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G586 - G591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Fornai, C Blandizzi, R Colucci, L Antonioli, N Bernardini, C Segnani, B Baragatti, S Barogi, P Berti, R Spisni, et al. Role of cyclooxygenases 1 and 2 in the modulation of neuromuscular functions in the distal colon of humans and mice Gut, May 1, 2005; 54(5): 608 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Linden, K. A. Sharkey, W. Ho, and G. M. Mawe Cyclooxygenase-2 contributes to dysmotility and enhanced excitability of myenteric AH neurones in the inflamed guinea pig distal colon J. Physiol., May 15, 2004; 557(1): 191 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. T. T. L. Tjwa, J. M. Bradley, C. M. Keenan, A. B. A. Kroese, and K. A. Sharkey Interleukin-1{beta} activates specific populations of enteric neurons and enteric glia in the guinea pig ileum and colon Am J Physiol Gastrointest Liver Physiol, December 1, 2003; 285(6): G1268 - G1276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R Linden, K. A Sharkey, and G. M Mawe Enhanced excitability of myenteric AH neurones in the inflamed guinea-pig distal colon J. Physiol., March 1, 2003; 547(2): 589 - 601. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
