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Am J Physiol Gastrointest Liver Physiol 284: G604-G616, 2003; doi:10.1152/ajpgi.00069.2002
0193-1857/03 $5.00
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Vol. 284, Issue 4, G604-G616, April 2003

TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach

Young Mee Lee*, Byung Joo Kim*, Hyun Jin Kim, Dong Ki Yang, Mei Hong Zhu, Kyu Pil Lee, Insuk So, and Ki Whan Kim

Department of Physiology and Biophysics, Seoul National University College of Medicine, Seoul 110 - 799, Korea

We investigated which transient receptor potential (TRP) channel is responsible for the nonselective cation channel (NSCC) activated by carbachol (CCh) in murine stomach with RT-PCR and the electrophysiological method. All seven types of TRP mRNA were detected in murine stomach with RT-PCR. When each TRP channel was expressed, the current-voltage relationship of mTRP5 was most similar to that recorded in murine gastric myocytes. mTRP5 showed a conductance order of Cs+ > K+ > Na+, similar to that in the murine stomach. With 0.2 mM GTPgamma S in the pipette solution, the current was activated transiently in both NSCC in the murine stomach and the expressed mTRP5. Both NSCC activated by CCh in murine stomach and mTRP5 were inhibited by intracellularly applied anti-Gq/11 antibody, PLC inhibitor U-73122, IICR inhibitor 2-aminoethoxydiphenylborate, and nonspecific cation channel blockers La3+ and flufenamate. There were two other unique properties. Both the native NSCC and mTRP5 were activated by 1-oleoyl-2-acetyl-sn-glycerol. Without the activation of NSCC by CCh, the NSCC in murine stomach was constitutively active like mTRP5. From the above results, we suggest that mTRP5 might be a candidate for the NSCC activated by ACh or CCh in murine stomach.

transient receptor potential protein

* Y. M. Lee and B. J. Kim contributed equally to this work.




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