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Am J Physiol Gastrointest Liver Physiol 284: G1093-G1103, 2003; doi:10.1152/ajpgi.00454.2002
0193-1857/03 $5.00
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Vol. 284, Issue 6, G1093-G1103, June 2003

REPORT
Identification of a basolateral Clminus /HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> exchanger specific to gastric parietal cells

Snezana Petrovic1,2, Xie Ju1, Sharon Barone1, Ursula Seidler3, Seth L. Alper4, Hannes Lohi5, Juha Kere6, and Manoocher Soleimani1,2

1 Department of Medicine, University of Cincinnati, Cincinnati 45267; 2 Veterans Affairs Medical Center at Cincinnati, Cincinati, Ohio 45220; 3 Department of Medicine, University of Tubingen, 72076 Tubingen, Germany; 4 Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215; 5 Department of Medical Genetics, University of Helsinki Finland, 00014 Helsinki, Finland; and 6 Department of Biosciences at Novum, Karolinska Institute, 14157 Huddinge, Sweden

The basolateral Cl-/HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> exchanger in parietal cells plays an essential role in gastric acid secretion mediated via the apical gastric H+-K+-ATPase. Here, we report the identification of a new Cl-/HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> exchanger, which shows exclusive expression in mouse stomach and kidney, with expression in the stomach limited to the basolateral membrane of gastric parietal cells. Tissue distribution studies by RT-PCR and Northern hybridizations demonstrated the exclusive expression of this transporter, also known as SLC26A7, to stomach and kidney, with the stomach expression significantly more abundant. No expression was detected in the intestine. Cellular distribution studies by RT-PCR and Northern hybridizations demonstrated predominant localization of SLC26A7 in gastric parietal cells. Immunofluorescence labeling localized this exchanger exclusively to the basolateral membrane of gastric parietal cells, and functional studies in oocytes indicated that SLC26A7 is a DIDS-sensitive Cl-/HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> exchanger that is active in both acidic and alkaline pHi. On the basis of its unique expression pattern and function, we propose that SLC26A7 is a basolateral Cl-/HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> exchanger in gastric parietal cells and plays a major role in gastric acid secretion.

SLC26A7; AE2


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