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HORMONES AND SIGNALING

Gastrin-induced gastric adenocarcinoma growth is mediated through cyclin D1

¹Section of Gastroenterology, Boston University School of Medicine, Boston Medical Center, Boston 02118; ²Gastrointestinal Division, University of Massachusetts Medical School, Worcester, Massachusetts 01605; and ³Department of Oncology and the Lombardi Comprehensive Cancer Center, Georgetown University, Washington, District of Columbia 20057

Submitted 6 December 2002 ; accepted in final form 17 February 2003

Gastrin is a gastrointestinal (GI) peptide that possesses potent trophic effects on most of the normal and neoplastic mucosa of the GI tract. Despite abundant evidence for these properties, the mechanisms governing gastrin-induced proliferation are still largely unknown. To elucidate the mechanisms by which gastrin might influence mitogenesis in gastric adenocarcinoma, we analyzed its effects on the human cell line AGS-B. Amidated gastrin (G-17), one of the major circulating forms of gastrin, induced a concentration-dependent increase in [³H]thymidine incorporation of cells in culture, with the maximum effective concentration occurring with 20 nM G-17. This effect was significantly attenuated by the gastrin-specific receptor antagonist L-365260. In addition, we found that G-17 induced a significant increase in the levels of cyclin D1 transcripts, protein, and promoter activity. The results of these studies indicate that gastrin appears to exert its mitogenic effects on gastric adenocarcinoma, at least in part, through changes in cyclin D1 expression.

gastrin-17; AGS-B cells; proliferation

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