HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/2/G354    most recent 00422.2002v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Troost, F. J. Articles by Brummer, R.-J. M. Search for Related Content PubMed PubMed Citation Articles by Troost, F. J. Articles by Brummer, R.-J. M.

MUCOSAL BIOLOGY

New method to study oxidative damage and antioxidants in the human small bowel: effects of iron application

Departments of ¹Human Biology and ²Pharmacology, Maastricht University and ³epartment of Gastroenterology, University Hospital Maastricht, All Nutrition and Toxicology Research Institute Maastricht, 6229 ER Maastricht, The Netherlands

Submitted 26 September 2002 ; accepted in final form 24 April 2003

Iron may induce oxidative damage to the intestinal mucosa by its catalyzing role in the formation of highly reactive hydroxyl radicals. This study aimed to determine iron-induced oxidative damage provoked by a single clinical dosage of ferrous sulfate and to elucidate the antioxidant defense mechanisms in the human small intestine in vivo. A double-lumen perfusion tube was positioned orogastrically into a 40-cm segment of the proximal small intestine in six healthy volunteers (25 ± 5 yr). The segment was perfused with saline and subsequently with saline containing 80 mg iron as ferrous sulfate at a rate of 10 ml/min. Intestinal fluid samples were collected at 15-min intervals. Thiobarbituric acid reactive substances concentrations as an indicator of lipid peroxidation increased significantly from 0.07 µM (range, 0–0.33 µM) during saline perfusion to 3.35 µM (range, 1.19–7.27 µM) during iron perfusion (P < 0.05). Nonprotein antioxidant capacity increased significantly from 474 µM (range, 162–748 µM) to 1,314 µM (range, 674–1,542 µM) (P < 0.05). These data show that a single dosage of ferrous sulfate induces oxidative damage and the subsequent release of an antioxidant in the small intestine in vivo in healthy volunteers.

duodenum; epithelium; lipid peroxidation; small intestine; oxidative stress

This article has been cited by other articles:

				F. J. Troost, R.-J. M. Brummer, G. R. M. M. Haenen, A. Bast, R. I. van Haaften, C. T. Evelo, and W. H. M. Saris Gene expression in human small intestinal mucosa in vivo is mediated by iron-induced oxidative stress Physiol Genomics, April 11, 2006; 25(2): 242 - 249. [Abstract] [Full Text] [PDF]

				F. J. Troost, R.-J. M. Brummer, G. R. M. M. Haenen, A. Bast, R. I. van Haaften, C. T. Evelo, and W. H. M. Saris Gene expression in human small intestinal mucosa in vivo is mediated by iron-induced oxidative stress Physiol Genomics, April 11, 2006; 25(2): 242 - 249. [Abstract] [Full Text] [PDF]