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MUCOSAL BIOLOGY

Inflammatory reaction without endogenous antioxidant response in Caco-2 cells exposed to iron/ascorbate-mediated lipid peroxidation

¹Centre de Recherche, Hôpital Sainte-Justine and Departments of ²Nutrition, ³Pediatrics, and ⁴Biochemistry, Université de Montréal, Montréal, Québec, Canada H3T 1C5

Submitted 24 January 2003 ; accepted in final form 20 June 2003

To characterize the role of intestinal epithelial cells in mucosal host defense, we have examined endogenous antioxidant reactivity and inflammatory response in Caco-2 cell line. When differentiated Caco-2 cells were incubated with iron/ascorbate for 1–24 h, they exhibited increased malondialdehyde levels and decreased polyunsaturated fatty acid proportion in favor of saturated fatty acids. These modifications were accompanied with alterations in membrane fluidity and permeability. The oxidative stress did not induce changes in the antioxidant enzyme activity of superoxide dismutase, catalase, glutathione peroxidase, and glutathione transferase, or in cellular glutathione content. However, iron/ascorbate-mediated lipid peroxidation promoted inhibitor-B degradation and NF-B activation, as well as gave rise to IL-8, cyclooxygenase-2, and ICAM-1. These results support the importance of oxidant/antioxidant balance in the epithelial cell inflammatory response.

oxidative stress; membrane transport; nuclear factor-B; cyclooxygenase-2; intercellular adhesion molecule-1

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