AJP - GI Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 285: G1225-G1234, 2003. First published July 31, 2003; doi:10.1152/ajpgi.00236.2003
0193-1857/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/6/G1225    most recent
00236.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (24)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xu, J.
Right arrow Articles by Soleimani, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xu, J.
Right arrow Articles by Soleimani, M.

MUCOSAL BIOLOGY

Identification of an apical Cl-/HCO3- exchanger in gastric surface mucous and duodenal villus cells

Jie Xu,1 Sharon Barone,1 Snezana Petrovic,1,2 Zhaohui Wang,1 Ursula Seidler,3 Brigitte Riederer,3 Krishnamurthy Ramaswamy,4,5 Pradeep K. Dudeja,4,5 Gary E. Shull,6 and Manoocher Soleimani1,2

Departments of 1Medicine and 6Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati 45267; 2Veterans Affairs Medical Center, Cincinnati, Ohio 45220; 3University of Tubingen, D-72076 Tubingen, Germany; and 4Department of Medicine, University of Illinois at Chicago, and 5Veterans Affairs Medical Center at West Side, Chicago, Illinois 60612

Submitted 22 May 2003 ; accepted in final form 28 July 2003

The molecular identity of the apical HCO3--secreting transporter in gastric mucous cells remains unknown despite its essential role in preventing injury and ulcer by gastric acid. Here we report the identification of a Cl-/HCO3- exchanger that is located on apical membranes of gastric surface epithelial cells. RT-PCR studies of mouse gastrointestinal tract mRNAs demonstrated that this transporter, known as anion exchanger isoform 4 (AE4), is expressed in both stomach and duodenum. Northern blot analysis of RNA from purified stomach epithelial cells indicated that AE4 is expressed at higher levels in mucous cells than in parietal cells. Immunoblotting experiments identified AE4 as a ~110- to 120-kDa protein in membranes from stomach epithelium and apical membranes from duodenum. Immunocytochemical staining demonstrated that AE4 is expressed in apical membranes of surface cells in both mouse and rabbit stomach and duodenum. Functional studies in oocytes indicated that AE4 functions as a Cl-/HCO3- exchanger. These data show that AE4 is an apical Cl-/HCO3- exchanger in gastric mucous cells and duodenal villus cells. On the basis of its function and location, we propose that AE4 may play an important role in mucosal protection.

anion exchanger isoform 4; chloride/bicarbonate exchange; parietal cells; mucous cells; acid injury


Address for reprint requests and other correspondence: M. Soleimani, Division of Nephrology and Hypertension, Dept. of Medicine, Univ. of Cincinnati, 231 Albert Sabin Way, MSB G259, Cincinnati, OH 45267-0585 (E-mail: manoocher.soleimani{at}uc.edu).




This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
D. Heitzmann and R. Warth
Physiology and Pathophysiology of Potassium Channels in Gastrointestinal Epithelia
Physiol Rev, July 1, 2008; 88(3): 1119 - 1182.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Y. Akiba, M. Mizumori, P. H. Guth, E. Engel, and J. D. Kaunitz
Duodenal brush border intestinal alkaline phosphatase activity affects bicarbonate secretion in rats
Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1223 - G1233.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
W. T. Nickell, N. K. Kleene, and S. J. Kleene
Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium
J. Physiol., September 15, 2007; 583(3): 1005 - 1020.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. E. Simpson, C. W. Schweinfest, G. E. Shull, L. R. Gawenis, N. M. Walker, K. T. Boyle, M. Soleimani, and L. L. Clarke
PAT-1 (Slc26a6) is the predominant apical membrane Cl-/HCO3- exchanger in the upper villous epithelium of the murine duodenum
Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G1079 - G1088.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H. Ishiguro, W. Namkung, A. Yamamoto, Z. Wang, R. T. Worrell, J. Xu, M. G. Lee, and M. Soleimani
Effect of Slc26a6 deletion on apical Cl-/HCO3- exchanger activity and cAMP-stimulated bicarbonate secretion in pancreatic duct
Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G447 - G455.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Mizumori, J. Meyerowitz, T. Takeuchi, S. Lim, P. Lee, C. T. Supuran, P. H. Guth, E. Engel, J. D. Kaunitz, and Y. Akiba
Epithelial carbonic anhydrases facilitate PCO2 and pH regulation in rat duodenal mucosa
J. Physiol., June 15, 2006; 573(3): 827 - 842.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. W. Freel, M. Hatch, M. Green, and M. Soleimani
Ileal oxalate absorption and urinary oxalate excretion are enhanced in Slc26a6 null mice
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G719 - G728.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Hentschke, M. Wiemann, S. Hentschke, I. Kurth, I. Hermans-Borgmeyer, T. Seidenbecher, T. J. Jentsch, A. Gal, and C. A. Hubner
Mice with a Targeted Disruption of the Cl-/HCO3- Exchanger AE3 Display a Reduced Seizure Threshold
Mol. Cell. Biol., January 1, 2006; 26(1): 182 - 191.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
N. McDaniel, A. J. Pace, S. Spiegel, R. Engelhardt, B. H. Koller, U. Seidler, and C. Lytle
Role of Na-K-2Cl cotransporter-1 in gastric secretion of nonacidic fluid and pepsinogen
Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G550 - G560.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Xu, J. Henriksnas, S. Barone, D. Witte, G. E. Shull, J. G. Forte, L. Holm, and M. Soleimani
SLC26A9 is expressed in gastric surface epithelial cells, mediates Cl-/HCO3- exchange, and is inhibited by NH4+ (Report)
Am J Physiol Cell Physiol, August 1, 2005; 289(2): C493 - C505.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. E. Simpson, L. R. Gawenis, N. M. Walker, K. T. Boyle, and L. L. Clarke
Chloride conductance of CFTR facilitates basal Cl-/HCO3- exchange in the villous epithelium of intact murine duodenum
Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1241 - G1251.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
O. Furukawa, M. Hirokawa, L. Zhang, T. Takeuchi, L. C. Bi, P. H. Guth, E. Engel, Y. Akiba, and J. D. Kaunitz
Mechanism of augmented duodenal HCO3- secretion after elevation of luminal CO2
Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G557 - G563.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Allen and G. Flemstrom
Gastroduodenal mucus bicarbonate barrier: protection against acid and pepsin
Am J Physiol Cell Physiol, January 1, 2005; 288(1): C1 - C19.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. R. Gawenis, H. Hut, A. G. M. Bot, G. E. Shull, H. R. de Jonge, X. Stien, M. L. Miller, and L. L. Clarke
Electroneutral sodium absorption and electrogenic anion secretion across murine small intestine are regulated in parallel
Am J Physiol Gastrointest Liver Physiol, December 1, 2004; 287(6): G1140 - G1149.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.